| Dendrobium candidum Chinese herbal medicine is a fresh or dry stalk of Dendrobium candidum,which has long history of medicinal health care in China.The ancient Taoist medical classic “ Dao Zang ” listed Dendrobium candidum as the first of “nine major grasses”.Dendrobium candidum has varied active ingredients,including polysaccharides,glycosides,Alkaloids,amino acids,trace elements and so on.Polysaccharides are the main active ingredient of Dendrobium candidum,which are the highest content.Dendrobium candidum has various skin care effects such as moisturizing,increasing cell activity,and anti-oxidation.As the top grade medicinal material,Dendrobium candidum has been welcomed by consumers,but the market of Dendrobium candidum is confusing.This article according to 2015 edition of "Chinese Pharmacopoeia" on the quality inspection of Dendrobium candidum bar,and optimize the extraction process of Dendrobium candidum polysaccharides,design and preparation emulsion of Dendrobium candidum,and the quality of emulsion related indicators,which provide ideas and research for the development and promotion of traditional Chinese medicine cosmetics basis.The research contents of this paper are shown as below:1)According to Dendrobium candidum quality standards in the "Chinese Pharmacopoeia" 2015 edition,testing items include:cross section identification,TLC identification,mannose and glucose peak area ratio,moisture,ash,alcohol extract content determination,content detection,polysaccharide content and mannose content.The results showed that the cross-section and TLC identification consistent with Dendrobium candidum traits.The water content was(11.15±0.36)%;the ash content was(3.14±0.06)%;the alcohol extract content was(8.43±0.28)%;the polysaccharide content was(45.05±1.24)%;and the mannose content was(29.14±0.01))%;peak area ratio of mannose and glucose was(2.63±0.04),which in line with the 2015 version of the “ Chinese Pharmacopoeia” related indicators.2)The effect of Dendrobium candidum polysaccharide content on the extraction rate of polysaccharides was investigated under the conditions of different ratio of liquid to material,extraction temperature and extraction time.The level of each factor was determined,and a three-factor and three-level simulation analysis experiment was conducted to determine the minimum.Multiply fitting to establish a regression model.Taking into account the stability of production and energy consumption factors,consider choosing the extraction temperature 90°C,the extraction time 2h,and the material-liquid ratio 66.5ml/g as the actual value of the parameter optimization value.It can be seen in the surface profiler that the conditionextraction rate is greater than 46% is larger and the surface is flatter,so it can be foreseen that the extraction is more stable at the time of production,and the actual results in the test also verify this point.3)According to the basic principles of cosmetic research and development,preliminary design of emulsion prescriptions,optimization of the amount of compound preservatives used,and inspection of microbiological content in products according to the microbiological test method in the 2015 edition of the "Safety Cosmetics Specifications".The results showed that heat-resistant Escherichia coli,Pseudomonas aeruginosa,and Staphylococcus aureus were all not detected,and the total number of colonies was less than 10.Considering the preservative efficacy and safety,the final selected preservative-optimized formula were phenoxyethanol 0.7%,EHG 0.3%,and methylparaben 0.1%.4)According to the 2015 “ Cosmetic Safety Risk Assessment Guidelines ”(Draft for Comment)on cosmetic risk assessment,the assessment results show that phenoxyethanol,ethanol,and hydroxyphenyl methyl ester in the raw materials may be brought into the risk substances according to their specifications and Dosage,no security risk.5)The stability and physicochemical parameters of the emulsion were investigated.The results showed that the p H of the sample was 6.20±0.15;the viscosity of the sample was(2800±102.3)mpa·s,which couldbe passed through heat and cold resistance,cold and heat cycles,and centrifuge tests.6)Inspection of the hygienic indicators in the emulsion products showed that the heavy metal content and various indicators of the microorganisms met the national standards.Prickly skin irritation results showed that the lotion was non-irritating.7)Evaluation of the moisturizing effect of the emulsion sample shows that the sample can quickly increase the MMV after being smeared and absorbed,and has a strong short-term moisturizing performance within two hours.After repeated application,perseverance can improve the moisturizing and lasting properties of the skin.The sample can restore the MMV value to a good level. |
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