| Gene therapy has long been held as having the potential to become a front line treatment of various genetic disorders.However,the direct delivery of nucleic acids to correct a genetic disorder has numerous limitations owing to the inability of naked nucleic acids?DNA and RNA?to traverse the cell membrane.Recently,messenger RNA?Mrna?based delivery has become a more attractive alternative to DNA due to the relatively easier transfection process,higher efficiency and safety profile.As with all gene therapy,the central challenge remains the efficient delivery of nucleic acids intracellularly.In this paper,a novel architectural terpolymer poly?DMAEA-co-DSDA-co-UDA?has been synthesized from via single electron transfer living radical polymerization?SET-LRP?,a type of Cu0&CuII-mediated controlled/living radical polymerization.By the similar polymerization,a series of bipolymers,terpolymers were synthesized to be used as control groups so that it is easy to analysize that the funtion of each component of the copolymer and to study the influence on material properties because of different feed ratios.The structure of polymers could be characterized by gel permeation chromatography?GPC?,nuclear magnetic resonance?1H-NMR?,transmission electron microscopy?TEM?and dynamic light scattering?DLS?.It is confirmed that this polymer does have an archtecture of single-chain cyclization with diameter of hundreds of nanometers and is positively charge,which shows the potential of being used for mRNA delivery.Subsequently,a series of cell experiments were performed,including electrophoresis,green fluorescent protein assay?GFP protein expression?,Luciferase assay?Luciferase transfection?,and AlamarBlue?cytotoxicity assay,which helps to evaluate the transfection efficiency and toxicity of synthesized polymers and finally confirmed that this kind of polymers as non-viral vectors for mRNA delivery have the advantages of high transfection efficiency and low cytotoxicity. |
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