Structure And Cytotoxicity Of 3,5-bis(1-imidazole)-pyridine Manganese,Zinc And Copper Complexes

As we all know,cancer has always been a major bottleneck in the medical field,and seriously threats people’s health.Thus,the development of a new anticancer drug from the perspective of coordination chemistry is of great significance.N-heterocyclic metal complexes have an intriguing,complicate and stable structure,play a significant role in the coordination chemistry,and have received many anti-cancer drug researchers attention.Designing and construction of metal-involved supramolecular architectures based on crystal engineering are currently of interests in the field of supramolecular chemistry and material science,not only by their intriguing structure,but also their potential applications as luminescence,catalysis and potential applications in biology,particularly in modern medicine.Multidentate ligands such as polycarboxylate and N-heterocyclic ligands are widely used in designing and controlling synthesis of these functional compounds.Recently,N-heterocyclic ligands have gained considerable attention due to their abundant coordination modes and functional properties,as well as hydrogen-bonding donors and acceptors under solution conditions.Transition metal complexes play a vital role in nucleic acid chemistry due to their diverse applications such as sequence specific binding,structural probes and therapeutic agents.In the last decades,an increasingly number of compounds containing transition metal ions such as platinum and ruthenium have shown to be active against cancer.These drugs have poor selectivity toward their biological target and high toxicity.In this paper,Three novel coordination polymers,[Mn（L）₂·（H₂0）₂]_n（1）,[Zn(L₂·（H₂0）₂]_n（2）,[Cu·L·（H₂0）₃]_n（3）were synthesized by 3,5-bis（1-imidazoly）pyridine under hydrothermal conditions.1.The crystal structures of the three complexes were determined by single crystal X-ray crystallography.2.UV-vis and fluorescence spectroscopy displays that the ability that coordination polymers bind to calf thymus DNA（CT-DNA）.3.Agarose gel electrophoresis assay exhibits the capacity of metal complexes to cleave the pBR322 plasmid DNA.4.Molecular docking shows that the complex 1-3 can interact with the special functional DNA,which makes the double helix DNA unspin and embed into the base pair.5.The apoptosis ability of HeLa cells induced by complex 1-3 was determined by flow cytometry,and color photographs were obtained by fluorescence inverted microscope.These images could clearly show the apoptosis inducing effect of metal complexes on human cervical cancer HeLa cells from morphological changes.The results showed that the complex 1-3 could interact with DNA,thereby affecting the stability of DNA and inducing apoptosis of HeLa cells.It can be used as an anticancer drug for future development.