The Preliminary Optical Properties Of NIR-ii Fluorescent Dyes And Their Quenchers

In optical imaging,high target(tumor)-to-backgrovund ratios(TBR)improve both the sensitivity and specificity.And the appearance of NIR smart probes makes higher TBR possible.In the study,we want to develop new NIR-II fluorescent dyes and their quenchers,providing reference for the development of new NIR smart probes.We want to use CH1055 for the development of smart probes for its higher spatial resolution,deeper penetration depth,excellent in vivo clearance properties and high signal-to-background ratios.After many steps of reactions,CH1055 is obtained.We have also explored the synthesis of a compound containing triazoles,but its synthesis is difficult and products are complex.Although many methods have been tried,the target product can’t be obtained.At last we choose to lay it aside temporarily.While the analogue of the target compound is obtained and preliminary experiments indicate that Lewis acids may affect the light absorption of this kind of compounds.In our future experiments we will study it further.Two different polymers have been designed and synthesized,and we expect using them as quenchers.By referring to literatures,a small molecular thienoquinoid compound is designed and obtained.Unexpectedly,preliminary experiments indicate that it’s a NIR-II fluorescent dye,which is of great meaning to the development of new fluorescent probes with better imaging quality.We will do more research on it,expecting using it for small animal imaging research.About the polymer we will continue its synthesis in the future.And at the same time,the synthetic route of the thiadiazoloquinoxaline polymer is explored and it’s obtained.Preliminary optical studies show that its properties of light absorption are in agreement with what were reported in the literature.Later the connection of CH1055 with the synthesized thiadiazoloquinoxaline polymer through a small molecule is tried.Preliminary optical studies show that the polymer does have the effect of quenching CH1055.And they may be expected to be used for the construction of new smart probes.While the successful connection of them still needs further verification and study.