The Method To Determine The Cyanide Content In Hevea Brasiliensis Seeds And Study On The Detoxification Process And Stability Of Hevea Brasiliensis Seeds’ Slurry

Hevea brasiliensis seeds is rich in plant protein, unsaturated fatty acids, vitamins and other nutrients. And it also has many functions such as disease prevention, anti-aging function. Considering the toxic ingredients in Hevea brasiliensis seeds such as cyanide and unstable oil protein milk system, it makes Hevea brasiliensis seeds not be studied. In orther to make full use of this resource of our country, in this paper the studies of the method to determine the cyanide content in Hevea brasiliensis seeds and the method to determine the Hevea brasiliensis seeds emulsion preparation, study the detoxification process of removing cyanide from Hevea brasiliensis seeds’ and the stability of Hevea brasiliensis seeds emulsion. The main test results were as follows:(1)Explore different detoxified methods on the residual cyanide in Hevea brasiliensis seeds. Used the the isonicotinic acid-pyrazolone spectrophotometric method and the isonicotinic acid-pyrazolone spectrophotometry with exogenous β-glucosidase to detected the contents of the cyanide. The results showed that the isonicotinic acid-pyrazolone spectrophotometry with exogenous β-glucosidase detected the contents of the cyanide is significantly higher than the former. Through the design of response surface method to determine the optimal condition was that treatment time for 3.35 h, soak temperature of 45.06 ℃, pH of 5.56 and the additive amount of P-glucosidase 24.11 U/100 mL. The prediction of cyanide content is 28.55 μg/g. The recovery was 85.02%～92.04%.(2) In order to examine the effect of protein containing and solid content on the stability coefficient. On the results of single factor, the optimal conditions for the orthogonal experiment. The experimental results for beating temperature was 80 ℃,beating time was 8min, beating pH was 8.0 and the rate of Hevea brasiliensis seeds and water was 1:15,in this condition,Hevea brasiliensis seeds protein extraction is 79.87% .(3)Through single orthogonal experiment and the design of response surface method,study the detoxification process of removing cyanide from Hevea brasiliensis seeds’ slurry.The first method for detoxification was boiling, after the single factor experiment of the boiling time get the optimal condition of removing cyanide for 20 min and the actually maximum rate of detoxification in Hevea brasiliensis seeds’ slurry will be reached to 90%.The second method for detoxification was citric acid combined with boiling, after orthogonal test to determine the optimal condition was that treatment time for 10 h and in’this process, changing the soak water every 2 h, soak temperature of 40 ℃ , water addition 4 times of the Hevea brasiliensis seeds mess and the actually maximum rate of detoxification in Hevea brasiliensis seeds’ slurry will be reached to 99.1%. The third method for detoxification was β-glycosidase enzymes combined with boiling,after the design of response surface method to determine the optimal condition was that treatment time for 1 h, soak temperature of 45.1 ℃, pH of 5.15 and the additive amount ofβ-glucosidase enzymes 15 U/400 mL. The actually maximum rate of detoxification in Hevea brasiliensis seeds’ slurry will be reached to 99%.(4) Studied the influence of the emulsifier, thickening agent and chelator on Hevea brasiliensis seeds emulsion stability, To further determine the impact of these factors on the Hevea brasiliensis seeds emulsion stability conditions. Results showed that when the raw material had 25% oil, the Hevea brasiliensis seeds emulsion stability is the best. Select stabilizer formula for: PGMS:SE15=6:4, 0.1%; Guar Gum:XG=6:4, 0.09%; sodium hexametaphosphate 0.05%,in this condition the emulsion stability coefficient is 93.58%.