The Manufacturing And Biomedical Application Of Hydrogel Fiber Based On Electrospinning

Tissue engineering has developed rapidly in the field of biomedicine in recent years,especially in wound repair,drug testing and other application scenarios.The ideal scaffold for tissue engineering needs to be able to simulate the environment of extracellular matrix,which puts forward strict requirements for material selection and scaffold manufacturing process.As for materials,natural biomaterials such as gelatin and hyaluronic acid have good biocompatibility,but their unstable properties and poor processability limit their further application.In terms of manufacturing process,the key of the construction of biomimetic extracellular matrix is to select appropriate teclhnology to obtain micro-nanofibers since macro-nanofibers have high porosity and large specific surface area.In general,micro-nanofi bers should be in appropriate diameter and can achieve orderly arrangement at micro-scale.Among many technologies,electrospinning has been rapidly developed in related fields in past few years and now is regarded to be a promising method to manufacture tissue engineering scaffolds in the future.In this research,the manufacturing technology of tissue engineering scaffolds was investigated using electrospinning technology based on a new hydrogel material GelMA with good biocompatibility and stable and controllable properties.The improvement of spinnability of GelMA was taken as the starting point.PEO and GelMA were blended in solution together for mixed spinning,which effectively improved the electrospinning performance of GelMA.Then a three-dimensional motion platform was designed and built.The preparation process of micro-nanofibers in both far and near field conditions was systematically studied,and the effects of factors such as distance,voltage and flow rate on the diameter and morphology of hydrogel fibers were emphatically explored.The macro and micro-scale disorderly arranged fibrous diaphragms were obtained under far-field conditions,and the macro-ordered and micro-disordered tissue engineering scaffolds were fabricated with PCL template.Fiber patterns and tissue engineering scaffolds arranged orderly on the micro-scale were obtained under near-field direct writing conditions.SEM results show that the prepared fibers have porous structure on the micro scale.FTIR analysis and degradation experiments show that PEO can be effectively removed by water immersion after crosslinking so the main component of the final fiber structure is GelMA.Finally,the biocompatibility of the scaffolds was investigated.Endothelial cells were cultured in vitro using the hydrogel fiber tissue engineering scaffolds.The biocompatibility of the scaffolds was evaluated by cell growth and proliferation.The results showed that cells can grow well on hydrogel fibers,and the original morphology and function of endothelial cells were maintained.