Separation Of Erythromycin A And Erythromycin C By Simulated Moving Bed Process

Erythromycin antibiotics are the third largest class of antibiotics in the world and have a wide range of applications in clinical medicine.In the fermentation of erythromycin,in addition to the target product erythromycin A,erythromycin C having a structure similar to that of erythromycin A is also produced.Due to the low biological activity and high toxicity of the components,there is a strict limit on the content of this erythromycin impurity in the International Pharmacopoeia.And the solvent extraction method in modern industry cannot selectively separate the erythromycin component.In this paper,the separation of erythromycin A and erythromycin C was studied by simulated moving bed chromatography.The main contents of this paper are as follows:The resolution of erythromycin A and erythromycin C was studied by liquid chromatography.The stationary phase used was C18 filler and the mobile phase was methanol/water/tetrabutylammonium hydroxide.In this chromatographic separation condition,erythromycin A and erythromycin C achieved complete separation.The competitive a dsorption equilibrium of erythromycin A and erythromycin C on the stationary phase was determined experimentally,and the experimental data were well fitted by the Com-Langmuir model.The experimental results show that the adsorption of erythromycin by the stationary phase filler is physical adsorption,and the adsorption process is an exothermic process.The adsorption and separation of erythromycin A and erythromycin C on a single column were studied.The model parameters such as void fraction,axial diffusion coefficient and mass transfer resistance coefficient were determined by.experiments.The mass transfer diffusion model of erythromycin A and C components on the column was established.The finite element orthogonal configuration method is used to numerically solve the model,and a stable numerical solution is obtained.The adsorption-desorption curve of erythromycin on the column was determined experimentally.Compared with the model results,the accuracy of the mathematical model was verified.A mathematical model for the separation of erythromycin A and erythromycin C by simulated moving bed chromatography was established.The model results were compared with the experimental results to verify the reliability of the SMB model.According to the triangle theory,the SMB separation zone was designed.The impurity component erythromycin C was successfully removed by SMB,and the purity of erythromycin A was finally increased from 87.97%to 95.80%.