Template-based Preparation Of Porous Silica Films And Their Applications In Protein Analysis

Protein imprinting is an emerging technology for protein separation detection.However,due to the large molecular sizes,the complexity of the structure and the variable conformation,which causes problems such as poor selectivity and poor mass transfer in protein-imprinted polymers.Molecularly imprinted polymers completely avoid these disadvantages.This paper aims at the limitations of current protein-imprinted technology.Based on preparation of porous silica films by template method.We prepare thin films with good selectivity and mass transfer capacity by using a sacrificial template method.The main work includes the following two aspects:1.Preparation of porous silica films using polystyrene microspheres and bacteria as templates:(1)preparation of self-supporting porous silica films using PS microspheres as templates.we use gas-liquid interface self-assembly method and sol-gel technology combined with the preparation of controllable,ordered microporous silica film.Then,self-assembly method is used to introduce carbon nanotubes film into the porous silica film to improve the mechanical stability of the silica film;(2)We use the bacterium as template and sol-gel technique in aqueous environment to prepare silica films with controlled morphology and thickness.2.Template-based preparation of porous silica films for protein analysis:(1)Preparation of porous silica films for protein-imprinted studies using bacteria as template.We use bacteria as a sacrifice template and couple protein molecules(GFP and CYC)to the surface of bacteria.Sol-gel technique in aqueous environment is used to prepare selective protein-imprinted film.We use GFP as a template protein,which qualitatively analyze of protein-imprinted film by using Fluorescence microscopy.We use CYC as a template protein to quantitatively analyze protein-imprinted film by using Uv-visible spectrophotometry,which include the adsorption capacity,adsorption kinetics,the selectivity binding of films.The results of the study show that the protein-imprinted films we prepared have better imprinting capacity,higher imprinting factors and good selectivity.(2)Preparation of porous silica films using PS microsphere as template,nano-gold is introduced into porous structure and the selective capture experiment of 100nm aminated microspheres is performed,which provides ideals for preparation of selective filtration membranes.