Study On The Contamination Of Typical Antibiotic Resistance Genes(ARGs) In Agricultural Soil And The Soil-air Migration

Antibiotic resistance gene(ARGs)is a new kind of pollutant,which widely exists in soil,water,air and other environmental media.This thesis systematically investigated the current situation of antibiotics and ARGs in farmland soil pollution of Yangtze River Delta(YRD)and 20 provinces in greenhouse and open-field soils of China for the fitst time.The ARGs soil-air migration and its influencing factors were studied.Theses researches attempted to provide some basic data for understanding the interface behavior of ARGs and controlling its soil pollution.The main results are as follows:(1)The concentration range of quinolone,tetracycline and sulfonamide antibiotics in soils of YRD is 4.5-2010 ng/g and doxycycline and ciprofloxacin have higher potential ecological risks.The average concentrations of antibiotics were 428 ng/g and 193 ng/g,and the pollution abundance of ARGs were 1.15×10-7-9.78×10-2 and<LOD-4.92x 10-2 copies/16S rRNA copies in greenhouse and open-field soils of Chian,respectively.The concentration of antibiotics is high in the east and north of China.The pollution level of antibiotics and ARGs was significantly higher in greenhouse soils than those in the open-field soils(p<0.05).The levels of ARGs in the greenhouse and open-field soils were affected by soil antibiotics and class ? integrons,and the soil in the greenhouse was more significantly affected by ARGs.(2)Soil ARGs enter the air environment through particulate matter.The soil-air migration trend of ARGs in highly polluted soil is more obvious,and the increase of soil moisture content will reduce the soil-air migration of ARGs.As the increase of soil moisture content will reduce the suspension of particulate matter and increase the soil ARGs abundance,when the moisture content increases to 20%,the trend of the decrease of ARGs soil-air migration slows down.