Study Of Curcumin Inhibits Liver Cancer Cells By Surface Enhanced Raman Spectroscopy

Raman scattering spectroscopy is a detection method that is convenient,fast,requires fewer samples,does not damage the sample,has high specificity and good repeatability.However,ordinary Raman spectroscopy has the disadvantages of weak signal strength and high detection limit.Surface-enhanced Raman scattering(SERS)spectroscopy remedy this disadvantage while retaining the advantages of Raman scattering spectroscopy.In recent years,SERS has been widely used in biotechnology and medical research related fields.Curcumin has various medical applications and has therapeutic effects on many cancers,including liver cancer.In recent years,the research work on curcumin has become more and more extensive and in-depth.In this paper,surface-enhanced Raman scattering technique was applied to study the inhibition of human hepatoma cells by curcumin,and the molecular information implied in SERS spectra was analyzed.The SERS spectral data of different samples were classified,and the accuracy of classification results was examined.The research work is as follows:Culture normal Lo2 cells,extract cells and their secretions as samples,add nano-silver colloids,dry,and then detect the mixture’s SERS spectrum.Analyze the spectral peaks in each spectrum and compare the difference between the Lo2 cell secretions and the culture solution spectra.Speculate the changes in the material of the liver normal cell culture process,use the principal component analysis(PCA)method to reduce the dimension,and combine the linear discrimination(LDA).Classification,and do the posterior probability and the subject’s work curve for the classification result to check the accuracy.Using the same method,hepatoma cells HepG2 were cultured,and the cells and their secretions were mixed with silver gel and subjected to SERS spectrometry.Spectral data were used to infer material changes in the process of hepatoma cell culture.The spectra of different cells or different cell secretions were compared to find out the characteristics of HepG2 cells and HepG2 cell secretion.Using curcumin as a cell proliferation inhibitor,liver normal cells Lo2 and hepatoma cells HepG2 were incubated at different concentrations and for different periods of time.The number of viable cells in each group was determined by MTT assay,and curcumin was calculated for the two cells according to the absorbance value.Effect work and do a simple analysis.Surface-enhanced Raman spectroscopy was performed on Lo2 cells,HepG2 cells,and their cell secretions after incubation with curcumin.The differences in SERS spectra between the same cells or cell secretions without curcumin plus curcumin were compared and classified using PCA-LDA.