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Study On The Preparation Of Chitosan Oligosaccharides By Enzymatic Method And The Function Of Its Health Food

Posted on:2019-12-15Degree:MasterType:Thesis
Country:ChinaCandidate:P Q GuoFull Text:PDF
GTID:2381330575470944Subject:Biochemistry and Molecular Biology
Abstract/Summary:PDF Full Text Request
Background:Chitosan oligosaccharide(COS)was a low molecular weight products which was soluble in water,more powerful,and more biologically active,and it obtained by degradation of chitosan,and its efficacy is dozens of times of chitosan,including immune regulation,hypolipidemic and hypoglycemic,protecting liver,anti tumor and heart and lung function improvement or other functions.Method:In this paper,the COS were prepared by enzymatic method.The optimal enzymatic hydrolysis conditions and the optimal enzyme were determined by single factor test and orthogonal optimization.Pectinase was used to degrade chitosan.The COS less than 3000 Da were prepared by membrane separation and spray drying;And then,two kinds of COS capsules(A and B)were prepared by compound design using COS with a molecular weight of 1200 Da(polymerization degree of 6)as the main raw material,and evaluate the function.The conclusions are as follows:The degradation effect of Pectinase on chitosan is better than that of cellulase and Chitanase;The COSs were prepared by using pectinase,and finally,the COSs with molecular weight of 1907 Da were obtained.Functional evaluation of COS capsules(A):In the experiment of carbon clearance in mice,the phagocytic index of high dose group was significantly higher than that of control group,indicating that COS capsule(A)could improve carbon clearance ability of mice to a certain extent.In the experiment of mouse ear swelling,the ear swelling degree in the middle and high dose group was significantly higher than that in the control group,indicating that the experimental results of chitosan swelling capsule(A)in the ear swelling of cell immune function were positive.In the serum hemolysin test of humoral immunity:Number of antibody of three dose group is significantly higher than the control group(p<0.05),showed that the COS capsule(A)in mice serum hemolysin test results were positive.Conclusion:the COS capsule(A)has the function of enhancing immunity.Functional evaluation of COS capsules(B):The results of Total cholesterol(TC)in rat serum showed that the TC of three dose groups was significantly lower than that of TC of high fat control group,indicating that COS capsule(B)had a positive effect on decreasing serum total cholesterol.The results of Triglyceride(TG)in rat serum showed that compared with the TG in the high fat control group,the TG level in the low and high dose group decreased significantly,indicating that the COS capsule(B)decreased the serum triglyceride result positively.The results of High-density lipoprotein cholesterol(HDL-C)detection show that the levels of HDL-C in the three dose groups were higher than those in the high fat control group,and the HDL-C level in the middle dose group was significantly higher than that in the high fat control group.Conclusion:the COS capsule(B)has the function of reducing blood lipid.
Keywords/Search Tags:Enzyme hydrolysis, COSs, capsules, enhanced immunity, auxiliary hyperlipidemia
PDF Full Text Request
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