Hydrolysis Of Bamboo Cellulose By Cellulases From Marine-Derived Fungi Strains

Penicilium imranainum is a marine-derived halophilic fungus that was studied as potential microbial producers of cellulases under liquid-state fermentation.The cultivation parameters（salinity,pH and temperature）and the performance of the cellulases in the hydrolysis of cellulose from bamboo were evaluated.The best cultivation time in liquid-state fermentation for producing cellulases was 5 days for all of the strains,which exhibited its highest filter paper activity（FPU）after 7 days.After optimizing pH and temperature,activities of 1.9 and 2.8 FPU g^-1（of substrate）was obtained.The hydrolysis of cellulose was studied using cellulose fraction of bamboo,with or without alkali and any other pretreatment.Independent of the fungi strain,untreated bamboo was resistant to saccharification.After the treatment,the degree of saccharification was 81%in P.imranianum and 58%in A.Niger.The hydrolysis by cellulases from A.Niger and P.imranianum produced 56%and 81%saccharification in the hemicellulose and cellulose,respectively.This fungus strain exhibits significant potential for the production of fermentable sugars resulting from the hydrolysis of cellulose from bamboo,mainly after Combine pretreatment with higher pH is 9 and with higher temperature is 60℃as compared to A.Niger.This research demonstrated that bioethanol from bamboo can be just as,if not more competitive than bioethanol from other lignocellulosic resources provided that certain criteria are fulfilled.