Construction And Bioaugmentation Efficiency Of Bacterial Agent For Purification Of Black Odor Water

Black odor water has the characteristics of high concentration of organic matter,low dissolved oxygen,low redox potential,low transparency and high ammonia nitrogen.The treatment of urban black odor water is a very complex matter.There are many sources and influencing factors of pollutants.The treatment methods generally include pHysical,chemical and biological methods.Physical,chemical and biological methods,pHysical and chemical methods for treating black and odorous water are expensive,and chemical methods can cause secondary pollution.Biological methods mainly treat black odorous water through microbial agents,improve black odor and water quality,and cost.It is low in implementation and simple in implementation.It has been practiced in some areas of China and has achieved ideal results.In this paper,high-efficiency COD-degrading bacteria and aerobic denitrifying bacteria were screened out from the sediment of a pond in Yixing.They were compounded with pHospHorus-removing bacteria and Deodorizing Bacteria preserved in the laboratory,purified the artificial black and odorous water,and studied on the film-forming of the compounded bacteria agent,and selected the best film-forming material.Firstly,29 strains of COD-degrading bacteria were screened from the sediment of a pond in Yixing by beef paste peptone starch plate,numbered 1?2?3...27?28?29.The removal rates of COD and NH₄⁺-N by these 29 strains were determined by adding them to artificial black odor water.The removal rates of COD and NH₄⁺-N by21 strains were the highest,reaching 60.39%and 19.63%.Twenty aerobic denitrifying bacteria were screened out by bromothymol blue plate?BTB?with the numbers of N1?N2?N3...N18?N19?N20.The removal rates of NH₄⁺-N and TN of these 20strains were determined by adding them to DM medium.The removal rates of NH₄⁺-N and TN of N12 strain were 98.7%and 91.44%,respectively.Through pHysiological and biochemical experiments and 16SrDNA sequencing,21 strains were identified as Bacillus aryabhattai strain and N12 strains as Pseudomonas stutzeri strain.Then the screened COD efficient degrading bacteria 21 and aerobic denitrifying bacteria N12 were compounded with the depHospHorizing bacteria DP4 and Deodorizing Bacteria TG4 preserved in the laboratory.The compounding agent was added into the artificial black odor water.The optimum combination was determined as TG4+DP4+21+N12.The removal rates of COD,NH₄⁺-N,TN and TP were 84.11%,38.4%,30.93%and 23%,respectively.The odor threshold was reduced from 800 to200.The optimal ratio is 1:1:1:2.The removal rates of COD,NH₄⁺-N,TN and TP are86.04%,87.92%,68.22%and 49.31%respectively.The odor threshold is reduced from800 to 100.The optimum purification conditions of the compound microbial agent were 30?,7.0 pH and 10%dosage of microbial agent.Finally,the combined microbial agents were placed on the three different fillers of the enamel-type packing,the combined packing and the elastic packing.The SEM was used to compare the filming conditions of the three kinds of fillers on the three kinds of fillers.The COD,NH₄⁺-N,TN and TP values of the artificial black odor water treated by three kinds of microbial agents within 30 days were measured.The braided fillers had the fastest film-forming speed,the largest amount of bacteria and the best purification effect.The best braided tape filler was selected to replace the artificial black odor water with the actual black odor water.The removal effect of the pollutants from the actual black odor water by the composite bacteria filler was investigated.