Preparation Of A Combined Drug Delivery System Based On Gold Nanorods And Its Antitumor Effect

Despite the development of science and technology,the level of modern medical treatment has been significantly improved,the forms of treatment for cancer are diversified,and radiotherapy and chemotherapy,as well as targeted precision treatment,have greatly improved the cure potential of cancer.However,with the rapid pace of modern life and the increasing pressure on people’s lives,the incidence of malignant tumors is not decreasing,and there is no very effective means for the treatment of malignant tumors.It is seriously endangering human health.In recent years,gold nanorods have been used as biomolecular probes for early tumor labeling,cancer cell localization and detection,biomedical detection and imaging,imaging and photothermal therapy based on the principle of photothermal conversion of heavy metals.The integration technology and other fields have extensive research and application,and have made significant breakthroughs.There is a big change to make the treatment of tumors from single to comprehensive,from non-specific to precise positioning to intelligently kill cancer cells.At present,functionalized gold nanorods as nanomaterials for loading anti-tumor drugs have become a research hotspot.The further functional application of gold nanorods will become an effective means for the diagnosis and treatment of malignant tumors.Among many nano drug-loaded materials,general metal materials have only one characteristic absorption peak.While gold nanorods are special,with two absorption peaks,one transverse surface plasmon resonance(TSPR),and a wavelength of 520 nm in the visible light region.The other longitude surface plasmon resonance(LSPR)is in the near-infrared region between 600 and 1000 nm.The treatment window of the biological tissue is between 700 and 1000 nm,so the near-infrared light in this wavelength range is not absorbed and can penetrate the tissue surface into the tissue.Furthermore,gold nanorods can convert light energy into heat energy,which provides a theoretical possibility for specific personalized photothermal therapy targeting tumors.Therefore,the subject has been studied as follows: First,prepare gold nanorods of appropriate size and conform to the biological tissue treatment window.Gold nanorods with an average particle size of 54.9 nm,a zeta potential of +20.5 mV,and a longitudinal plasmon resonance absorption peak of 721 nm were synthesized by seed growth method;in order to remove the biocompatibility and facilitate the next experiment,The surface of the gold nanorods is carboxylated by HS-mPEG-COOH by group replacement to remove the toxicity of CTAB,increase biocompatibility;then activate the carboxyl group by DCC/DMAP to pass the esterification condensation reaction.Curcumin was linked to gold nanorods,and the first drug was successfully linked to the carrier to produce GNRs-mPEG-Cur.Finally,the thermosensitive liposome was used to encapsulate the gold nanorods linked to curcumin with paclitaxel.Together,the biocompatibility is improved,the solubility of the drug is increased,the circulation time of the drug in the body is prolonged,and the passively targeted GNRs-mPEG-Cur@PTX@Lip(GPC@PTX@Lip)nano drug delivery system is realized by releasing the drug in the temperature response of the tumor site.The MTT method was used to observe the effect of GPC@PTX@Lip nano-preparation on the growth of cancer cells.The results showed that the preparation had certain lethality to tumor cells,and under the action of the photothermal effect and long liposome circulation in gold nanorods,the cancer cells have good targeting and photothermal treatment effects.Thereby achieving targeted delivery of drugs and synergistic effects on combined administration of tumor tissues to greatly enhance the therapeutic effect and reduce the side effects of improving toxicity.The in vitro safety evaluation experiment confirmed that the prepared GPC@PTX@Lip preparation had no hemolysis,indicating that the blood compatibility was good.The above results indicate that the GPC@PTX@Lip prepared by the subject has a suitable particle size,uniform dispersion in water,obvious anticancer effect,and relatively stable in blood,and is a good clinical application in the future.