Carbon And Nitrogen Removal Characteristics Of ABR Decarbonization-Nitrosation Coupled With ANAMMOX

In order to solve the problems existing in nitrite-anaerobic ammonia oxidation process,the integrated process of ABR decarbonization and nitrosation coupled with anaerobic ammonia oxidation was put forward.In order to provide an effective technical reference for the engineering application of this process in the treatment of actual domestic sewage,the single-stage operation of ABR carbon removal,CSTR nitrification and anaerobic ammonia oxidation,the control strategy of the combined process,the control law of the coupled process and the microbial ecological characteristics of the functional bacteria were emphatically studied.Specific research contents and results are as follows:1.In order to explore the nitrogen removal performance of the long-term storage nitrosation flocculent sludge,nitrosation flocculent sludge which had been stored at 4?for a long-term of 10 months was inoculated in the continuous stirred tank reactor?CSTR?to investigate its recovery performance of activity.The high throughput sequencing technology of MiSeq was also employed to analyze the structure of microbial community before and after the reactivation.Results showed that under the control of DO0.4-0.8mg·L^-1,pH value about 8 and temperature?30±1??,the long-term storage nitrosation flocculent sludge could be quickly reactivated in 15 days,both the removal efficiency of ammonia nitrogen and the accumulation ratio of nitrite nitrogen were higher than 90%.Meanwhile,the color of the sludge quickly restored from early inoculation of grey to brown,the sludge volume index?SVI?was decreased quickly and both the mixed liquor volatile suspended solids?MLVSS?/mixed liquor suspended solids?MLSS?and extracellular polymer substances?EPS?increased significantly.With the recovery of nitrosation performance,anaerobic and heterotrophic bacteria was washed out,the relative abundance of ammomia-oxidizing bacteria,such as Nitrosomonas ect.,increased significantly,while the growth of nitrite-oxidizing bacteria such as Nitrospria was inhibitedselectively.Nitrosation flocculent sludge after long-term storage could be inoculated to achieve rapid start-up of partial nitrification and benefit the practical application of partial nitrification process.By inoculating anaerobic ammonia oxidation sludge in laboratory anaerobic ammonia oxidation seed sludge reactor,the removal performance of NH₄⁺-N and NO₂^--N by anaerobic ammonia oxidation sludge was improved by gradually shortening HRT.In addition,another ABR reactor was built to achieve a certain effect of ABR carbon removal,which was prepared for the subsequent study of ABR carbon removal-CANON process.2.In order to promote the application of anaerobic ammonium oxidation?ANAMMOX?to treat municipal wastewater,coupled nitrosation with ANAMMOX and added ABR decarbonization system at the front of it to build the ABR decarbonization-nitrosation coupled with ANAMMOX to treat municipal wastewater.The high throughput sequencing technology of MiSeq was employed to analyze the structure of microbial community in sludge.Results showed that the average effluent COD concentration of carbon removal system was 120mg·L-¹and the subsequent nitrosation system and ANAMMOX system would not be adversely affected by the effluent COD.Under the control of matching effluent of nitrosation with ABR carbon removal system 2:1 as influent of ANAMMOX will meet the ratio of NO₂^--N and NH₄⁺-N matrix to ANAMMOX about 1:1.The total nitrogen removal rate of the integrated reactor was 88%-90%,and the COD of the effluent was 20-40mg·L^-1.At the same time,after the experiment,the number of?-Protebacteria class in the nitrosation system closely related to denitrification increased,Sphingobacteria class with the function of enhancing the growth rate of microorganisms and enhancing the rate of denitrification was significantly increased in the ANAMMOX system.The nitrogen and carbon of municipal wastewater could be stable and efficiently removed by the ABR Decarbonization-Nitrosation coupled with ANAMMOX process.3.In order to promote the application of anaerobic ammonium oxidation?ANAMMOX?to treat municipal wastewater,coupled nitrosation with ANAMMOX and added ABR decarbonization system at the front of it to build the ABR decarbonization-nitrosation coupled with ANAMMOX to treat municipal wastewater.The high throughput sequencing technology of MiSeq was employed to analyze the structure of microbial community in sludge.Results showed that the average effluent COD concentration of carbon removal system was 120mg·L^-1and the subsequent nitrosation system and ANAMMOX system would not be adversely affected by the effluent COD.Under the control of matching effluent of nitrosation with ABR carbon removal system 2:1 as influent of ANAMMOX will meet the ratio of NO₂^--N and NH₄⁺-N matrix to ANAMMOX about 1:1.The total nitrogen removal rate of the integrated reactor was 88%-90%,and the COD of the effluent was 20-40mg·L^-1.At the same time,after the experiment,the number of?-Protebacteria class in the nitrosation system closely related to denitrification increased,Sphingobacteria class with the function of enhancing the growth rate of microorganisms and enhancing the rate of denitrification was significantly increased in the ANAMMOX system.The nitrogen and carbon of municipal wastewater could be stable and efficiently removed by the ABR Decarbonization-Nitrosation coupled with ANAMMOX process.