The Preparation Of Magnetic Functional Nickel-cobalt Alloy Nanorings For The Application Of Cancer Diagnose And Magnetic Induction Therapy

In recent years,cancer is a serious threat to people’s health.Existing treatments,such as surgery,radiotherapy and chemotherapy,have serious side effects and often bring pain to patients.Magnetic induction hyperthermia（MIH）can effectively kill tumor cells,which become the focus of research in tumor hyperthermia.And because of the characteristics of minimally invasive,non-toxic,small side effects,has been recognized"green therapy"by the international medical community.In chapter 3 we used acetylacetone complexes as precursor,polyvinylpyrrolidone（PVP）as the surfactant to prepare high magnetic cobalt nickel alloy rings（NiCo）by high temperature hydrothermal synthesis method.Then NiCo were modified with bovine serum albumin（BSA）and polylysine（PLL）by electrostatic adsorption,and the functional nickel cobalt alloy rings（NiCo@BSA@PLL）were successfully synthesized.Surface potential,particle size,X-ray diffraction,TG and FTIR were systematically analyzed.The size of a single particle is about 40 nm,and the size of a single ring is about 300 nm.The SAR value of NiCo@BSA@PLL 2 mg/mL can reach to 272 W/g.SQUID characterization showed that the saturation magnetization of NiCo@BSA@PLL was 30 emu/g,which reflected the high magnetism and superparamagnetism.For the application of cancer therapy,we developed a NiCo@BSA@PLL nanorings mediated tumor diagnosis and treatment integration strategy by combining magnetic resonance imaging（MRI）and MIH.Three cell lines were used to verify the biosafety of the material in vitro.Secondly,the killing effect of the material on tumor cells was verified in vitro,and the results showed that the killing effect of NiCo@BSA@PLL on HepG2 and 4T1 cells was up to 71%and 84%,respectively.Finally,after intratumoral injection of the NiCo@BSA@PLL into mice,MRI results showed obvious enhanced T₂ MRI effect.And after exposing to alternating magnetic field,the tumor region temperature could reached to 54^oC,indicating an excellent tumor inhibition potential.Futhermore,we applied the mixture of NiCo@BSA@PLL and silk protein to prepare functional hydrogel NiCo@BSA@PLL@Silk（NCBP@Silk）by strong ultrasonic.This injectable hydrogel also has good heating effect under alternating magnetic field and good biocompatibility,which is expected to serve as a new medium for magnetic induction hyperthermia.