Study Based On Fluorocarbons Of The Treatment Of Pulmonary Diseases

Lung is a tremendously significant part of human body.Acute lung injury caused by hypoxia and lung tumor are two very serious lung diseases.For these two diseases,we have carried out research based on the excellent characteristics of fluorocarbons.In view of the above two kinds of lung diseases,we choose PFCs as the research object and PLGA as PFOB carrier.Some researches show that perfluorocarbon(PFCs)has many excellent properties,especially the strong ability of dissolving oxygen(40%).Therefore we have developed two kinds of fluorocarbon nanoparticles for the protection and treatment of the lungs.1.PLGA-PEG/PFOB emulsion was prepared successfully.The cell experiments were used to characterize the effect of composite emulsion on cells.The emulsion was proved that has good biocompatibility and can promote the growth of A549 cells to a certain extent under low oxygen concentration.It also plays a significanted role in recovery from hypoxia reoxygenation experiments.The evaluation of the expression level of ROS and HIF-1 alpha confirmed the balance mechanism between cell recovery and apoptosis.Then the saline lavage acute lung injury model and hypoxia environment model have been builded to proved that the emulsion have obvious effect on improving pulmonary ventilation.Lung histological sections and lung compliance further show that it can be used as a surfactant to reduce surface tension and improve lung compliance and permeability.2.we have established PFOB-PLGA-IR780 nanocapsule loaded with photosensitizers and fluorocarbons,which can improve oxygen concentration of tumors.And the effect of PDT also can be improved because of PFOB.The photothermal curves in vitro and in vivo show that the photothermal conversion performance is very good.At the same time,the growth of A549 cells and lung tumors both have been inhibited by the combination therapy.The addition of PFOB increased the therapeutic effect of PDT and significantly enhanced the therapeutic effect.Through these experiments,we can prove that PFC materials can be used as an important research object in the treatment of lung cancer and acute lung injury.