Preparation And Properties Of Cooperative Transport Composite Nanospheres

Objectives Cetyltrimethylammonium bromide was used to prepare calcium carbonate hollow nanospheres with appropriate size by changing reaction conditions.Calcium carbonate hollow nanospheres were loaded with p53 gene.Calcium carbonate hollow nanospheres were modified with sodium alginate to improve their stability and loaded with doxorubicin hydrochloride.Then the properties of the nanospheres were preliminarily studied.Methods Cetyltrimethylammonium bromide was dissolved,then self-assembles into approximate spheres in aqueous solution,through dropping appropriate concentration of sodium carbonate solution and calcium chloride solution to form calcium carbonate hollow nanospheres.The reaction conditions were studied by using different feed ratios of sodium carbonate solution and calcium chloride solution,different rotational speeds,and different temperatures of reaction system,.The optimum reaction conditions of calcium carbonate hollow nanospheres were obtained.The Calcium carbonate hollow nanospheres were characterized by particle size analysis and electron microscopy morphology analysis.The hollow nanospheres prepared in the optimum preparation conditions were modified and synergistically loaded drug.The drug loading and encapsulation efficiency were determined.The drug distribution experiments in vivo were carried out to evaluate the properties of the nanospheres.MTT method was used to measure the cell inhibition rate and observe the cell morphology.Results 1 Under the same amount of cetyltrimethylammonium bromide and other reaction conditions,the calcium carbonate hollow nanospheres with the concentration of sodium carbonate and calcium chloride at 0.02 mol/L were the smallest,while the calcium carbonate hollow nanospheres with the reaction speed of 1000 r/min were the smallest when the amount of cetyltrimethylammonium bromide and other reaction conditions were unchanged.When the amount of alkyl trimethylammonium bromide and other reaction conditions remain unchanged,the particle size of nanospheres in space-time cavity is the smallest at 20 C.2 The potential of calcium carbonate hollownanospheres modified by sodium alginate was-27.20(+0.75 m V),which significantly increased the stability of the composite nanospheres.3 The drug loading of the carrier increased gradually with the increase of reaction time and stabilized at 6 h.The encapsulation efficiency was 38.55%and the drug loading was 7.71%.4.The distribution of composite nanospheres in animals showed that the content of adriamycin hydrochloride detected in liver was the highest,while that in kidney was slightly higher.Composite drug-loaded nanoparticles had a certain liver targeting effect.5 The inhibition rate of drug-loaded nanoparticles was significantly higher than that of drug-loaded nanoparticles alone,and the results of cell morphology were consistent with the results.Conclusions 1 The optimum condition for calcium carbonate hollownanospheres to control the feed ratio and concentration is 20℃ 1000r/min.2 The morphology of calcium carbonate hollow nanospheres was the best by transmission electron microscopy,and obvious cavity structure could be observed.Calcium carbonate hollow nanospheres are more stable.Modified nanospheres not only increase their stability,but also achieve synergistic drug delivery.3 Compound drug-loaded nanoparticles have obvious targeting and tendency in liver and spleen tissues.The results of cell inhibition rate measured by MTT method were consistent with those of cell morphology observation,which proved that the composite drug-loaded nanoparticles had synergistic drug delivery effect.Figure 14;Table 16;Reference 84...