Study On The Preparation Of Drug Delivery System Based On Cyanine Dyes For Chemo And Photothermal Combination Antitumor Therapy

Near-infrared(NIR)light-induced photothermal agent-based stimuli-responsive materials have attracted great interest from researchers.However,the high smart release with precise control by NIR light is not yet well established because of the lack of or inadequacy of intelligent release,such as premature release of drug and/or photothermal agent.Herein,we put forward a novel and convenient strategy to synthesize cyanine dye functionalized polymeric materials,this multifunctional design strategies will provide a novel strategy to attain high smart drug delivery for precise cancer therapy.1.We initiatively synthesized methylacryloyl functionalized cyanine(MACyanine).Then,by taking use of RAFT polymerization,p(NIPAM-co-MACyanine-co-MCMEMA)copolymer with different feed ratios were obtained.Besides,the copolymer reacted with hydrazine.Finally,the DOX was attached to the hydrazide-functional copolymer via formation of hydrazone bond to obtain the p(NIPAM-co-MACyanine-co-MCMEMA)-g-DOX copolymer.The polymeric materials had NIR light-responsive photothermal effect and photostability.Meanwhile,The nanovehicles had finely pH/NIR light-controlled drug release and enhanced cell killing under NIR laser irradiation by comparison with free DOX.Additionally,the intrinsic fluorescence of the polymeric is help for imaging-guided photothermal therapy.This investigation is helpful for the nanovehicle as a therapeutic delivery system for chemo and photothermal combination antitumor therapy.2.We initiatively synthesized ATRP chain transfer agent based on cyanine dyes(BIBCyanine).By taking use of RAFT polymerization,PGMA-co-PDMDEA copolymer were obtained.Besides,the copolymer reacted with ethylenediamine.Finally,The PEG was attached to the copolymer to obtain the target product PEG-p(EGMA-co-DMDEA).The copolymer disassembled in acidic conditions as the hydrophobic ortho esters hydrolyzed into hydrophilic alcohol groups.PEG segments were designed to improve stability of the copolymer in the blood circulation.The design enabled the copolymer degradation step by step in acidic milieu,thus boosting the release of encapsulated drugs at the targeted site.Besides,The copolymer had finely NIR light-responsive photothermal effect.The nanocarrier displayed a multifunctional propertie of NIR light and pH-sensitivity,which provided a new way to develop novel multifunctional drug delivery systems.