Ectoine Improves Salt Tolerance Of Microbial Cells In Microbial Fuel Cells

Microbial fuel cell(MFC)coupling technology for electricity production and wastewater purification is a new technology.On MFC structure and operation with high production capacity has attracted considerable attention.Therefore,the coupling technology of MFC electricity generation and wastewater purification under conditions of high salt needs to solve the problem of inhibition of microbial growth and metabolism.In this paper,we added osmotic pressure compensation solute Ectoine to the salt-containing MFC substrate,and made MFCs high-efficiency electricity production under high salt.This paper provides a theory and method for the efficient production of electricity in high-salt substrate MFC using general electrogenic microbial flora(non-salt-tolerant or halophilic).The superior growth and electrogenic strain isolated and purified from the MFC anode microbial membrane was identified as Acinetobacter.sp AO1.When the NaCl concentration of the MFC substrate is increased(gradually increased from 0 to 60 g/L NaCI),the growth of the strain is inhibited,thereby reducing the electricity production.Under the same conditions of increasing MFC NaCl concentration,the electricity production of MFC anodes also decreased significantly.Adding Ectoine to improve the electricity production of high-salt matrix MFC:Adding 1 mmol/L of Ectoine to a high-salt matrix(30 g/L NaCl),the maximum output voltage of MFC is increased by 33.1 compared with the high-salt matrix without Ectoine.%,the average output voltage increased by 60.4%during the stable period,and the maximum power density increased by 52.1%.The maximum output voltage of A01 MFC increased by 45.6%,the average output voltage of the stable period increased by 51%,and the maximum power density increased by 39.9%.From the microbial diversity analysis of MFC anode biofilm with high salt matrix(30 g/L NaCl)added with 1 mmol/L of Ectoine,it can be seen that under this condition,the abundance of microbial microorganisms is high,which is beneficial to increase the production of MFC system.Electrical performance and removal efficiency of pollutants.The dominant bacteria of the anode biofilm:Proteobacteria(relative abundance 80%),y-proteobacteria of the Proteobacteria(72.20),and Acinetobacter(38.9%).