Preparation Of Polylysine Gene Delivery System And Its Application In Cancer And Cerebrovascular Diseases Therapy

Cancer and cerebrovascular diseases are both serious diseases that threaten human health.Due to the complexity of these diseases,some therapy may be restricted to use.It is difficult to completely cure these diseases by traditional treatments,and may cause serious complications.Therefore,it is imperative to study a safe and effective treatment.Gene therapy is a new method which can be used to treat many serious diseases like cancer and other major diseases.Constructing a safe and efficient gene vector is crucial in gene therapy.Cationic gene vectors have high transfection efficiency,good structure designability,low cost and no immunogenicity.However,most cationic gene vectors are only used for the treatment of specific disease.There are few universal gene vectors can be used for gene therapy of various diseases.In the second chapter of this paper,in order to construct a gene carrier with low toxicity and high efficiency,we use lysine as the raw material,introduce the disulfide bond which can be degraded,and prepare the degradable branched cationic gene carrier via ring-opening reaction.This gene carrier can be degraded in a reductive environment,and has low cytotoxicity and high transfection efficiency.Using this vector to carry pKillerRed,a photodynamic protein plasmid,to evaluate the in vitro gene therapy mediated photodynamic therapy for glioma,KillerRed protein can be successfully expressed in glioma cells and kill cancer cells effectively after light exposure.In the third chapter of this paper,in order to further expand the application of the biodegradable branched polycation in other serious diseases,we first have optimized its structure.Then,vascular endothelial growth factor plasmid pVEGF is deliveried by this vector.Polycation/pVEGF complex is coated on the surface of carotid artery stent to accelerate vascular reendothelialization and prevent in-stent restenosis.