| Xinjiang is one of the important agricultural products.Starting with the fermented food inXinjiang agricultural products,this paper discusses the pathogenic bacteria in kimchi:Staphylococcus epidermis;Halophile:Bacillus halophilus,halomonas was identified.Foodborne pathogenic bacteria is one of the important factors endangering human health for a long time,affecting the economic development of the region,and is one of the difficult problems to be solved by public health at present.For halophilic bacteria,halophilic bacteria is a kind of archaea,and has a high research value,for example,in kimchi it can decompose the casein well in food,improve the flavor of fermented foods,in addition,because of its efficient solution of salt ability,can well deal with industrial wastewater,so it has the very high economic benefits.However,for the southern Xinjiang region,first,the public health conditions are poor,the incidence of food-borne bacteria by mistake is high,and there is no good detection means for which bacteria can cause disease.Second,the southern Xinjiang is mostly saline and alkaline areas,can develop the local characteristics of bacteria,for agriculture or industrial development will be greater benefits.Therefore,this paper adopts the method of differential Raman and surface enhancement technology to detect Staphylococcus epidermidis,Bacillus halomonas,and obtain their unique fingerprint,so as to realize the purpose of rapid detection of bacteria.In the later stage,a microfluidics chip for microbial detection was preliminarily designed,which realized the function of collecting microbial samples in feces mode and attaching enhancer.The chip was used to identify the Raman peak of Staphylococcus aureus in raw mutton.The main research contents of this paper are as follows:（1）Characterization of gold and silver sol with ultraviolet spectrometer finally concluded that the optimal configuration method of gold and silver sol reinforcer was new preparation,with heating time of 45 minutes and pH of 7.（2）The identification of Staphylococcus epidermidis by time-of-flight mass spectrometry showed that staphylococcus epidermidis was highly credible in the detection of bacterial species;Bacillus halloysitus is highly reliable at the level of fungi.From this test,it can be seen that the time-of-flight mass spectrometry has the ability to identify a certain strain under the condition of unknown strain,which lays a foundation for the identification of three strains by using Raman in the later period.（3）Differential Raman and surface enhancement technology were used to detect the strains.It was proved that under the enhancement of gold and silver sol,the characteristic peak position information of Staphylococcus tabiformis was different except about 1460cm-1,and the enhanced position information of gold sol was better than that of silver sol.In this experiment,it was found that under the enhancement of aursol,bacillus halomonas and Bacillus halomonas were enhanced at 728cm-1,and the two halophilus had a common enhancement peak at 955cm-1.Moreover,the SERS spectrum of the three types of bacteria was significantly different,which could be directly identified through the spectrum.It was also found that the characteristic substances of archaea represented by Bacillus halomonas were enhanced at about 1521cm-1,while the characteristic substances represented by halomonas were enhanced at about 558cm-1,1401cm-1,which could be used as the basis for the identification of the two bacteria.（4）Through the preliminary design of the microfluidic chip,solve the chip surface enhancement agent attachment,rapid detection of fungi and other functions.The design and production process of the chip were summarized,and the detection of Staphylococcus aureus in agricultural products was completed by Raman spectrum.Six characteristic Raman peaks of Staphylococcus aureus were obtained and the Raman peaks of S.aureus were identified. |
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