Preparation Of Surfaces Based On Nanoparticle-Glycopolymer Complex And Their Applications

Surface immobilized glycopolymer plays an important role in the study of interactiones between saccharides and lectins or other proteins,bacteria and cells.The preparation of well defined glycopolymer via control/"living" polymerization is the premise of the above studies.Nanoparticle composited glycopolymer surface,with excellent synergistic performance,can greatly enhance physical and chemical properties of material’s surface,which is gradually becoming the research focus of surface immobilized glycopolymerIn this study,glycopolymers with pendent sugar moieties synthesized via control/"living"radical polymerization are used as the precursors.The physical and optical properties of silver nanoparticles(AgNPs)or carbon quantum dots(CQDs)composited glycopolymer surfaces are studied.Moreover,the abilities of adsorbing bioactive molecules as well as adhering and killing bacteria of the composited glycopolymer surfaces are also investigatedThe main topics of this study are as follows:1)In this part,we first synthesize various dopamine-containing glycopolymers(P1s and P2s)via a one-pot sunlight-induced reversible addition-fragmentation chain transfer(RAFT)polymerization.N-3,4-dihydroxybenzenethyl methacrylamide(DMA),2-(methacrylamido)glucopyranose(MAG)and/or methacrylic acid(MAA)are used as monomers.The results show that due to the surface adhering ability of catechol groups,the synthesized dopamine-containing glycopolymers can self-adhere to surfaces of different materials.The plenty of sugar moieties in glycopolymer coatings result in superhydrophilicities and antifogging properties of the coatings.After introducing carboxyl groups into the glycopolymer coating,excellent antimicrobial property can be endowed on basis of formed complex(Ag+ions and carboxylate group)and in situ created AgNPs,which result in antifogging and antimicrobial coatings.These antifogging and antimicrobial thin coating shows strong prospect in medical and optical devices,with extra benefits of avoiding potential pathogen infection in vitro or while storage.2)We choose MAG as the monomer to synthesize well defined glycopolymer(PMAG)via sunlight-induced RAFT polymerization.The apparent molecular weight of PMAG is 40300 and the Mw/Mn is 1.3,as characterized by SEC.The glycopolymer solution(3 wt%)is used to prepare films with thicknesses of about 50 nm by spin coating.Nano-patterned carbon quantum dots(CQDs)can be in situ formed by electron beam lithography at different doses.We find that the CQDs will emit fluorescences under the excitation of 405 nm wavelength.In addition,the specific adsorption abilities between nano-patterned glycopolymer CQDs and lectin(ConA-FITC)as well as microorganism(E.coli)are also studied.Finally,the abilities of CQDs to form AgNPs in situ are investigated.The results show that ConA-FITC,E.coli and in situ formed AgNPs tend to adhere on the outside of the CQDs,which may result from the cross-linked glycopolymers on the outside of the CQDs.