Basic Research On Application Of SO₃ Micro-thermal Explosion Technology In Extraction Of Chinese Medicinal Materials

This paper based on the relatively mature SO₃micro-thermal explosion technology of the research group,extracted the Chinese herbal medicines after pretreatment,which provided a new pretreatment method for improving the yield of the effective components and expanded the substrates processed by SO₃micro-thermal explosion technology.By observing the cinnamon bark before and after pretreatment by SO₃micro-thermal explosion technology,analyzing the chemical composition,content and functional groups of cinnamon bark extracts before and after pretreatment,and establishing the kinetic equation of cinnamaldehyde extraction before and after pretreatment.Box-behnken design carried out to optimize the factors that may be affecting the SO₃micro-thermal explosion combined with cinnamaldehyde extraction.The research contents and main conclusions are as follows:1. According to the difference of effective ingredients in Chinese herbal medicines,we researched Chinese herbal medicines such as coptis,scutellaria,liquorice,magnolia officinalis,cinnamon bark,ganoderma lucidum,rhubarb,andrographis paniculata,salvia miltiorrhiza and qinpi.The results showed that SO₃micro-thermal explosion technology could effectively improve the yield of the effective components of the Chinese herbal medicines,of which the yield of magnolia officinalis（honokiol）was the most increased significantly,with a maximum increase of 60.41±0.14%.2. The GC-MS,SEM,FTIR and micro-infrared imaging used to characterize the morphology of cinnamon bark before and after pretreatment,the components of the extract,functional groups and cinnamaldehyde residues,and establish cinnamaldehyde extraction kinetic equations before and after pretreatment.The results show that the SO₃micro-thermal explosion technology has no effect on the quality of cinnamaldehyde.The mechanism of the pretreatment on the cinnamon bark is that the SO₃vapor diffuses and reacts with the H₂O in the cinnamon bark,which releasing abundant heat to weaken the connection between the fibers and destroy the sieve tube of the cinnamon bark.Therefore,pretreatment facilitated the subsequent extraction of cinnamaldehyde.The establishment of the extraction kinetic equation proves that the pretreatment method not only improves the yield of cinnamaldehyde,but also greatly improves the extraction efficiency of cinnamaldehyde.3. Using response surface optimization to optimize the process parameters of cinnamaldehyde combined with SO₃micro-thermal explosion technology,the optimal conditions were obtained:pretreatment temperature 65℃,pretreatment time 60 min,soaking time 40 min and particle size 80 mesh.Under the optimal process,the optimal extraction rate was 3.32±0.07%,which was 85.47±0.27%higher than that obtained by direct extraction without treatment.