| In this paper,the waste ginseng residue from ginseng processing plant was used as the experimental raw material,and the compound enzyme method and twin-screw extrusion method were used to extract and optimize the dietary fiber in the waste ginseng residue,so as to increase the extraction rate and get better quality.To provide a theoretical basis for the development of new products in the future its basic properyies were determined.Meanwhile,pharmacological experiments were carried out on the extracted dietary fiber to provide data reference for the development and utilization of ginseng dietary fiber in the prevention and treatment of diabetes and antioxidation.In addition,the extracted soluble dietary fiber was used to make functional beverage,which makes ginseng dietary fiber have more extensive value in use.The main research results of this paper are as follows:(1)Protease and amylase were used to assist in the preparation and further extraction of insoluble dietary fiber(IDF),from ginseng residue to increase its extraction rate in ginseng residue so as to reduce the waste of raw materials.After the response surface assisted enzymatic optimization,the final optimal conditions were obtained as follows: the ratio of material to liquid was 1: 25g/m L,the amount of enzyme was 0.5%,the time of enzymatic hydrolysis was 1 hour,and the particle size was 80 mesh.Under these conditions,the extraction rate of IDF was 68.11%.(2)Cellulase and hemicellulase were used to assist in the preparation and further extraction of insoluble dietary fiber(IDF),from ginseng residue to increase its extraction rate in ginseng residue so as to reduce the waste of raw materials.After the response surface assisted enzymatic optimization,the final optimal conditions are as follows: the ratio of material to liquid is 1: 15g/m L,the amount of enzyme is 2%,the time of enzymatic hydrolysis time 1 hour,and the particle size is 40 mesh.Under these conditions,the extraction rate of IDF was 67.57%.(3)The optimal conditions of modification and optimization of soluble dietary fiber(SDF)of ginseng residue by twin-screw extrusion were as follows: temperature 140 C,rotational speed 130r/min,water content 40%.Under these conditions,the extraction rate of SDF was 34.5%.(4)Through the determination and comparison of the components of common ginseng dietary fiber and high quality ginseng dietary fiber,it can be concluded that the water holding capacity,oil holding capacity,expansibility and glucose adsorption capacity of the modified high quality ginseng dietary fiber are better than those of ordinary ginseng dietary fiber,which has a good value of comprehensive utilization.(5)The soluble dietary fiber(SDF)from optimized extraction and insoluble dietary fiber(IDF)were applied to animal experiments to explore the effect of ginseng dietary fiber on hypoglycemic function.Through the in vivo experiment,it concludes that the middle dose group of SDF and the middle dose group of IDF have the best hypoglycemic effect on experimental animals.(6)The optimized soluble dietary fiber(SDF)and insoluble dietary fiber(IDF)were applied to the antioxidant test in vitro.Through the scavenging effect on free radicals and the determination of reducing ability,it was found that the antioxidant capacity of SDF was stronger than that of IDF.(7)The modified high quality soluble dietary fiber was used to make functional beverage.Through many single factor and orthogonal experiments,the best scheme was determined as follows: according to the weight parts,8 parts of sorbitol,0.2parts of citric acid,0.25 parts of sodium hydroxymethylcellulose(CMC),0.25 parts of pectin,0.04 parts of vitamin C and 100 parts of soluble dietary fiber and distilled water were mixed to get the mixture.The final concentration of SDF in the mixture is 6mg/m L.Under such conditions,the taste and appearance of ginseng dietary fiber beverage are the best. |