Biodegradation Of Perchlorate From Water Using Hydrogen-basesd Membrane And Biofilm Reactor

Perchlorate is a kind of persistent toxic pollutants,there is a huge toxic to human body health.The traditional process is expensive,and easy to produce secondary pollution.Hydrogen-based Membrane Biofilm Reactor?MBfR?hollow fiber membrane microporous aeration oxidation and biological membrane method processing the water pollutant,is clean,no subsequent pollution,biological hydrogen production rate low,oxidation has been widely used in the water pollution governance.This subject adopts the shaker device for hydrogen autotrophic bacteria cultivation and domestication,and based on the experimental study on anaerobic sequencing batch type under the condition of microorganism with H₂ as electron donor ClO₄^-the feasibility of reducing water and its influencing factors.Results show that the hydrogen reduction of autotrophic bacteria is the main cause of ClO₄^-concentration attenuation,and out of the water in the process of reaction concentration of Cl^-concentration increase with the removal of ClO₄^-is between0.331-0.375,close to fully reflect theory than?0.356?,shows that ClO₄^-was completely restore to Cl^-;ClO₄^-reduction of the optimum temperature for 30-35?,the best pH=7.0-8.0;Coexistence of SO₄^2-and ClO₄^-,SO₄^2-had no significant effect of ClO₄^-reduction;ClO₄^-reduction rate increases with NO₃^--N concentrations of a downward trend,that NO₃^--N,reduction of ClO₄^-has obvious inhibitory effect;When the initial concentration of 1-9 mg/L,ClO₄^-has high removal rate,the reduction rate increases with the increase of initial concentration.At the same time,based on the hydrogen-based membrane biofilm reactor operation test,investigates the MBfR of ClO₄^-in water reduction and influencing factors,and establish the relationship between the electronic distribution and biological membrane reaction kinetics.Results show that the MBfR of ClO₄^-in water has good removal effect,to the reaction at the end of ClO₄^-removal rate close to100%;Increase the hydrogen partial pressure can promote ClO₄^-reduction,but even if the continuous increase of hydrogen partial pressure,also cannot make ClO₄^-removal rate is linear growth;Improve the inlet flow velocity and surface of ClO₄^-load increase rapidly,resulting in a loss reduction effect;ClO₄^-reduction of the optimal pH value of 7.0 to 8.0;With the increased concentration of NO₃^--N and NO₂^--N and ClO₄^-and standardized fluxes are declining,shows that both can inhibit ClO₄^-reduction;In TN the certain circumstances,the electron flux of NO₃^--N ratio is higher than the NO₂^--N and NO₃^--N concentration changes of ClO₄^-under low reduction dynamics series,showed the NO₃^--N to ClO₄^-reduction of inhibition was stronger than NO₂^--N.Finally,the domestication of raw sludge,sludge and sludge samples under different NO₃^--N concentrations of high-throughput sequencing and analysis of microbial population structure change and the law of succession.Results show that in shake flask sludge and hanging film sludge,deformation bacteria as the advantage bacterium group,give priority to with beta phylum deformation,followed by?-and?-deformation,phylum is mainly done by bacterial Methyloversatilis and Citrobacter ClO₄^-reduction;Under different NO₃^--N concentrations,?-deformation bacteria as the dominant fungi,followed by a certain amount of?-and?-deformation bacteria,the total content of three kinds of deformation with the increase of NO₃^--N concentrations showed a trend of lower after the first rise;At the same time,in different genera of microbes,Methyloversatilis,Zoogloea and Citrobacter dominant,both can also simultaneously ClO₄^-and NO₃^--N in raw water,three of the total amount of bacteria in NO₃^--N concentrations of 1 to 10 mg/L gradually rise,NO₃^--N concentrations continue to improve,a downward trend,conforms to the water concentration of NO₃^--N and ClO₄^-change rule.