| The separations of chiral compounds are widely studied.Chromatography is an important method of separation and detection.Accurate qualitative and quantitative analyses are often hindered in experiments.It is particularly important to optimize the chromatographic conditions for enantioseparations.The optical resolutions of some amino acids and chiral medicines are of great significance to the pharmaceutical and chemical industries.The thesis mainly includes the following aspects:（1）R-（3,3’-diphenyl-1,1’-dinaphthyl）-20-crown-6 was synthesized and coated on reversed phase C188 silica gel to make the stationary phase.The splits of chromatographic peaks of phenylglycine,p-hydroxyphenylglycine,and methionine on the columns were systematically studied.The experimental results show that pH of mobile phase,acidity of dissolved sample,injection volume,and different types of acids are able to effect the split of chromatographic peaks.（2）Synthesized amylose tri（3,5-dimethylphenylcarbamate）,it was coated on five different derivatized macroporous silica gels to prepare stationary phases.The resolution of five acidic chiral compounds on the five chiral columns were investigated with four mobile phases.When coated amylose tri（3,5-dimethylphenylcarbamate）on the silica gel bonded alkane chain,the better optical resolutions of acidic compounds may be obtained.The normal phase mobile phase is perfect for the enantioseparations of mandelic acid,DNB-leucine,and ketoprofen.Ibuprofen and flurbiprofen have better recognition under reversed phase mobile phase.（3）Five chiral columns were prepared by synthesized cellulose tri（4-methylbenzoate）coated on derivatized macroporous silica gels.The better chiral separations of acidic medicines can be reached on the columns prepared with aminopropyl derivatived silica gel.The enantioseparations of mandelic acid,DNB-leucine,and ketoprofen are suitable with normal phase mobile phase,reversely,ibuprofen and flurbiprofen are fit with reverse phase mobile phase. |
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