Synthesis And Analytical Application Of Copper Nanoclusters

Metal Nanoclusters（Mncs）,A Promising Luminescent Material,Have Been Used in the Detection of Ions,Molecular and Biomacro-Molecular,Fluorescent Imaging,Cancer Diagnosis and Treatment and Other Fields As New Fluorescence Sensors Due to Their Low Toxicity,Good Biocompatibility and High Rate.in This Paper,Three Kinds of Small Molecules Were Used As Ligands to Prepare Copper Nanoclusters with Different Properties,and Their Properties Were Analyzed by Means of Fluorescence,Ir and Uv-Visable Spectroscopy,Tem and Xps.Then,Fluorescence Sensor System for Small Molecular Substances and Metal Ions Were Constructed Based on the Quenching Mechanism.Moreover,It Were Applied to Cell Imaging.Chapter1:We Introduced the Characteristics,Synthesis Methods and Potential Applications of Copper Nanoclusters.Based on the Relevant Literature,the Research Ideas of This Paper Were Designed.Chapter2:the Water-Soluble Copper Nanoclusters（Capt@Cu Ncs）Were Synthesized by Thiol Small Molecule Captopril As Ligand.It Was Found That There Were Had A Good Response Between the Copper Nanoclusters and Hemin.and the Fluorescence Intensity of Cu Ncs Were Quenched with Incerasing the Concentration of Hemin,Which Had A Good Linear Relationship in the from0.05to42.0μMol/L.Therefore,A Simple and Reliable Detection Method for Hemin Was Constructed,and the Results Proved That the Quenching Mechanism Is Electrostatic Interaction.and the Method Was Applied to the Detection of Hemin the Human Blood Samples.Chapter3:Water-Soluble Copper Nanoclusters（Pgp@Cu Ncs）Were Synthesized by“One-Pot”with Penicillin G Potassium（Pgp）As A Protective Agent and Reducing Agent.the Results Showed That the Fluorescence Intensity of the Pgp@Cu Ncs（at474Nm）Decreased Significantly and the Isoemission Point Was Appeared at601Nm When the Certain Concentration of Doxorubicin Was Added.It Was Further Proved That the Fluorescence Intensity of the Copper Nanoclusters Exhibited A Goodworking Linear with the Concentration of Doxorubicin Ranges from1.0to35.0μMol/L and from45.0to100.0μMol/L,Respectively.Thus,the Sensing System for the Selective Detection of Doxorubicin Has Been Constructed Based on Fluorescence Quenching of the Cu Ncs.the Linear Range of the Method Was Wide and the Detection Limit Was18Nmol/L.in Addition,the Fluorescence Probe Can Quantitatively Detect Doxorubicin in Doxorubicin Hydrochloride for Injection and Be Successfully Applied in Confocal Imaging at Smmc7721Cells.Chapter4:the Vb₁@Cu Ncs with Low Toxicity and High Luminous Rate Was Synthesized by Using Thiamine Hydrochloride（Vb1）As Protective Agent and Reducing Agent,and Its Quantum Yield（Qy）Was²0.12%.Thiamine Hydrochloride Is A Small Sulfur-Containing Molecule That Has A Good Reduction Ability.and the Sulfur Atom in Thiazole Ring Was Easy to Interact with Metal Copper Ion.Thus,It May Be Formed Cu-N Coordination Bond Due to the N Atom of Pyrimidine Ring of Thiamine Hydrochloride Protonated.Moreover,It Was Found That Au³⁺Can Effectively Quench the Fluorescence of Cu Ncs.Tem Showed That the Larger Particle Size of Cu Ncs Appeared When the Addition of Au³⁺,Which Showed the Aggregation Happened Between Au³⁺and Cu Ncs.There Had A Good Linear Range of1.0⁵0.0μMol/L with the Concentration of Au³⁺.Moreover,the Applicability of Our Detection System Is Also Verifified by Analysis of Au³⁺in Various Water and the Imaging of Biological Cells.Chapter5:We Summarized the Application of Different Functional Copper Nanoclusters As Fluorescent Probes,and Look Forward to the Future Research Was Forecasted.