Correlation Study Of VEGF And IGF-1 In Serum, Aqueous Humor And Vitreous Of Proliferative Diabetic Retinopathy

Objective: This study is conducted to test the expression of VEGF and IGF-1 in serum,vitreous and aqueous humor of patient with proliferative diabetic retinopathy,and the correlation between these factors and proliferative diabetic retinopathy.Therefore,we can analyze the relationship between them and find new ways to treat PDR.Methods: 38 patients with proliferative diabetic retinopathy,retinal detachment,macular hole etc were selected to do vitrectomy in our hospital from December 2016 to October 2017.We detailed record the age,gender,medical history etc of these patients.And we measured the contents of VEGF and igf-1 in serum,aqueous and vitreous humor by enzyme-linked immunosorbent assay for further comprehensive analysis.Results: 1.There was no obvious difference in age and sex between PDR group and control group(P>0.05).2.(1)VEGF concentration in vitreous was significantly higher than in serum and they were positively correlated relationship(r=0.74,P<0.01).(2)There was no statistical differences between serum with aqueous humor and aqueous humor with vitreous at VEGF concentration in PDR group(P>0.05).(3)There was no statistical differences in serum,aqueous humor and vitreous of VEGF concentration in the control group(P>0.05).3.(1)There was no significant correlation of VEGF concentration at serum and aqueous humor between PDR group and control group(P>0.05).(2)The VEGF content in the vitreous of PDR group was significantly higher than that in the control group,and the difference was statistically significant(P<0.05).4.(1)There was significant difference between IGF-1 concentration in serum and that in vitreous of the PDR group(r=0.45,P<0.05).(2)There was no statistical differences between serum with aqueous humor and aqueous humor with vitreous at IGF-1 concentration in PDR group(P>0.05).(3)There was no significant correlation in serum,aqueous humor and vitreous of IGF-1 concentration in the control group and they has no statistical difference between each other(P>0.05).5.(1)There was no significant correlation of IGF-1 concentration at serum and aqueous humor between PDR group and control group(P>0.05).(2)The IGF-1 content in the vitreous of PDR group was significantly higher than that in the control group,and the difference was statistically significant(P<0.05).6.(1)There was no significant correlation between VEGF and IGF-1 in serum,aqueous humor and vitreous of PDR group(P>0.05).(2)There was no significant correlation between VEGF and IGF-1 in serum,aqueous humor and vitreous of control group(P>0.05).7.There was no significant correlation about sex of VEGF and IGF-1 concentration in serum,aqueous humor and vitreous(P>0.05).Conclusion: 1.The progression of PDR may increase the expression of VEGF and IGF-1 in vitreous.2.VEGF and IGF-1 in vitreous plays an important role in the progress of PDR.3.Age or sex did not affect the development of diabetic retinopathy.4.We cannot assess the degree of PDR lesion by test the concentration of VEGF in blood.