The Effect Of NSCs On The Proliferation And Differentiation Of Fetal Rat NSCs And Its Related Factors

Objective: to observe the dislocated qing nao yi yuan soup on fetal rat brain death after the differentiation of neural stem cells proliferation were studied,the influence of qing yi yuan brain soup experimental basis for prevention and treatment of ischemic cerebrovascular disease and ischemic cerebrovascular prevention research vision further.Methods:Purchased by animal experiment center of guangxi medical universi-ty health clean grade SD rats were 30,20 male and female 10,rat rat age of 10 to 12 weeks,weight 250-50 g,which randomly take 10 male SD rats were ivided into two groups: normal group and the qing yi yuan brain soup serum gr-oups,each group 5,only a day in at 9 am and 5 PM lavage distilled water or qing yi yuan brain soup twice,at 28 d line of sublingual vein blood of rats,res-pectively,the preparation of normal serum and serum of qing yi yuan brain soup-20 ℃ cold standby.10 surplus male SD rats,weight 250-50 g,random and 10 female rats cage let its mating,observe whether there is a white fine suppository form,if have the record of 0.5 d,to pregnant 12 d fetal rats,dislocated death,separation,preparation of rat embryonic neural stem cells.Establish neural stem cells in vitro model of low sugar low oxygen,and to give qing y-i yuan soup treatment,brain by immunohistochemical method to detect each group.Results: 1.compared with the blank group,reduced the number of neural stem cell proliferation,hypoxia model group difference was statistically significa-nt(P ﹤ 0.05),the expression of VEGF increased,BDNF and b FGF expression increases,for the protective performance.The difference is statistically sig-nificant(p﹤0.05).2.Compared with the hypoxic model group,the number of nerve stem cells in the normal serum group increased,and the difference was statistically significant(P < 0.05).VEGF expression increased,BDNF and b FGF expression dec-reased,and the difference was statistically significant(p < 0.05).3,compared with normal serum group,the number of neural stem cells in the brain yi yuan tang group was obviously increased,the difference was statistically significant(P ﹤ 0.05),the expression of VEGF increased,reduce BDNF and b FGF expression,differences were statistically significant(p ﹤ 0.05).Conclusion:Qingnaoyi yuansoup oflow sugarlowoxygenenvironment can pro motes the proliferation of rat embryonic neural stem cells differentiation effec t,its mechanism may be qing yi yuan brain soup related factors for promoting the growth of neural stem cells with a protective expression...