To Learn The Influence Of The Detemination Of Bilirubin By Means Of Diazo And Vanadate Oxidation In The Patient With Paraprotein

Objective :to study the effect of paraprotein in bilirubin measured by diazo method,vanadate oxidation and dry chemistry through bilirubin concentration basing on in the patients with paraprotein.Methods: The serum of 247 cases with paraprotein positive from Anqing Municipal Hospital from August 2015 to June 2017 was collected and divided into treatment groups: multiple myeloma(MM)group and non MM group.The serum of 50 case with negative paraprotein was collected as the control group.Each sample was analyzed by dry chemical method,diazonium method,and vanadate oxidation method.Compared with dry chemicalAnalyse the results of the determination of bilirubin in ways of diazo and vanadate was analyzed.Results:The differences of the bilirubin concentration by the means of diazonium and vanadate oxidation were statistically significant in the group of MM and non MM,while the differences were not statistically significant in the control group.The D-value of diazo method and dry chemistry were statistically significant in the MM group and control group,as in the groups of MM and non MM,meanwhile they were statistically significant in the non MM group and control group.The D-value of vanadate oxidation and dry chemistry in the MM group and control groupwere statistically significant,as non MM group and control group.the D-value of vanadate oxidation and dry chemistry in the groups of MM and non MM were not statistically significant.Conclusion:The total bilirubin concentration was affected by diazotization and vanadate oxidationa applied in the patients with paraprotein,while paraprote between MM and non MM cases had different effects on the determination of bilirubin by diazo method.