Association Of NF-?B Signal Transduction Pathway And Inflammation-related Gene Polymorphisms With HPV16,18 In Cervical Cancer

Objective:Cervical cancer is the most common malignancy in women,ranking second in the female reproductive system malignancy.Human Papilloma virus(HPV)infection,especially high-risk HPV infection,is a major risk factor for cervical cancer.HPV infection is present in over 90% of cervical cancer patients,with 40% to 60% HPV16.However,most women infected with HPV do not develop cervical cancer.It is only a sexually transmitted infection,and the body spontaneously eradicates HPV,and only a few eventually develop cervical cancer.This indicates that high-risk HPV infection is a necessary condition for cervical cancer deficiency and may have other significant risk factors.Therefore,further study of cervical cancer etiology and molecular epidemiology,molecular markers,the relevant signaling pathways to find timely and effective screening high-risk groups,and then take effective preventive measures.This topic PCR-RFLP technique was applied to 336 cases of cervical cancer cases and 304 cases of age matched normal cervical tissue in HR-HPV16,18 infection and NF-?B-94(ins/del ATTG),TNF alpha-308(G/A),IL-1? and T/C,IL-10-1082-G/A,IL-17-152(G/A)gene polymorphism detection,analysis of NF-?B signaling pathways and inflammatory factor related gene polymorphism and the relationship between cervical cancer susceptibility of HPV16,18.In order to explore the possible pathogenesis of cervical cancer and cervical cancer with the our country population of risks related to genetic susceptibility genes,and then screening high-risk groups or susceptible individuals,as to provide theoretical basis for disease prevention at the same time.Method:A total of 336 newly diagnosed patients with cervical cancer diagnosed by histopathology were selected as the case group and 304 patients(± 5 years old),sex matched non-tumor patients were selected as the hospital-based molecular epidemiology case-control study In the control group,the subjects were from the first people's hospital of yancheng,the second people's hospital of yancheng and the third people's hospital of yancheng,the case and healthy physical examination population;using polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP)NF-?B-94(ins / del ATTG),TNF-?-308(G / A),IL-1?-31 T / C,IL-10-1082 G / A,IL-17-152(G / A)Gene polymorphism was used to compare the risk of cervical cancer in different genotype carriers and high-risk HPV infection.The effects of age,age and birth rate on cervical cancer were analyzed by stratified analysis.Result:HR-HPV16/18DNA test results showed that HPV16,18 infection in cervical cancer and the control group the difference was statistically significant(p<0.05).NF-?B-94(ins / del ATTG)II,ID,DD genotype and genotype of GG,GA and AA in IL-10-1082 G / A were different in HPV16,18 positive and negative No statistically significant(p>0.05).There were significant differences in the genotypes of TNF-?-308(G / A)GG,GA and AA in HPV16,18 positive and negative groups.AA genotype is a susceptible genotype of HPV16 infection,increasing the risk of HPV16 infection;CA and AA genotype increased susceptibility to HPV18 infection.IL-1?-31 T / C carrying the-31 TT genotype is associated with an increased risk of developing cervical cancer in the individual-31 CC genotype.The TT genotype is a susceptible genotype of HPV16 infection and increases the risk of HPV16 infection.There was no significant difference in genotype of GG,GA and AA among IL-17-152(G / A)genotype in HPV16 positive group and negative group.AA genotype increased susceptibility to HPV18 infection.Conclusion:1.The positive rates of HPV16 and 18 infections in cervical cancer patients were significantly higher than those in the normal population,which can be used as an important method for early screening and risk assessment of cervical cancer.2.There was no correlation between NF-?B-94(ins/del ATTG)and IL-10-1082 G/A in women and cervical cancer caused by HPV16,18 infection.TNF-?-308(G/A),IL-1?-31T/C,and IL-17-152(G/A)may be associated with the occurrence of HPV16,18 infection and subsequent cervical cancer.