Study On The Effect And Mechanism Of Parecoxib Sodium Pretreatment On Postoperative Cognitive Dysfunction In Rats

Objective:Morris water maze test was used to determine the cognitive response of rats before and after operation and the effect of preadministration of parecoxib sodium on the cognitive function of rats after operation,and to explore the mechanism of the effect of preadministration of parecoxib sodium on cognitive dysfunction after operation in rats.Methods:Healthy male SD rats,aged 3-4 months,weight 250-300 g.Randomly divided into 4 groups: The control group(group D)was not given anesthesia and surgical treatment;the sham operation group(group J)was anesthetized but only bilateral carotid arteries were separated without clipping.The other rats who were anesthetized and underwent bilateral carotid artery occlusion for 60 minutes were divided into two groups.Group T was given intraperitoneal injection of parecoxib sodium 2 days before operation and the day of operation.Thesaline group(group Y)was given the same amount of normal saline at the same time point.Some of the rats were tested for spatial learning and memory by Morris water maze test 5days before operation and 6 days after operation.The other part of the rats did not carry out water maze test.The hippocampus was taken at 2 hours,1 day,3 days and 7 days after operation to determine the content of interleukin-1 β and TNF-α in hippocampus.Results:The rats in the Morris water maze test had no significant difference in the learning and memory ability at the same time points between the groups at each time point 5 days before the operation.Compared with the saline group(group Y)and T group in the first day after surgery,the time of finding the target platform was significantly shortened(P<0.05).Compared with the sham operation group(J group),there was no significant difference in the time between J group and T group(P >0.05).Compared with the control group(group D),there was no significant difference(P>0.05)in the time after 1 day after surgery between D group and T group.On the 6th day aftersurgery,compared with the group Y,the number of times that rats in group T crossed the target quadrant was significantly increased.(P<0.05).Compared with group J,there was no significant difference(P>0.05)in the 6 days after surgery for the group T.There was no significant difference in the number of crossing the target quadrant on the 6th day after operation between group T and group D(control group)(P>0.05).The changes of hippocampal IL-1β and TNF-α in rats were similar,both of which began to rise after surgery and peaked at the first day after surgery,and then began to decline.In group Y,the increase was the largest,and compared with T group,there was statistically significance on the 1st,3rd and 7th day after operation.There was no significant difference between group J and group T on the levels of hippocampal IL-1 βand TNF-α at the same time point(P>0.05).Conclusion:Bilateral carotid artery occlusion for 60 minutes can cause postoperative cognitive dysfunction in rats.Preoperative pretreatment of parecoxib sodium can improvepostoperative cognitive dysfunction in rats.Postoperative cognitive dysfunction rats showed a short period of hippocampal inflammatory response.Parecoxib sodium can reduce the levels of IL-1β and TNF-αin the hippocampus of rats with postoperative cognitive dysfunction.