The Cytotoxic Of Gold Nanoparticles With PPD On The Hep-2 Cells And The Inhibition Of Larynx Cancer In Vivo

PPD is an effective component extracts from ginseng,which has been proved to have the effect of inducing apoptosis and inhibiting the metastasis on several cancer cells.But its poor solubility and low bioavailability is always an issue.Nanomaterials provide a new platform for the development of new drugs combined with the advantages of passive targeting,higher penetration,higher loading rate,drug delivery and intracellular function.Gold nanoparticles is widely used because of its empty structure,large size,thin and solid shell.Using these unique advantages,it is used as a drug carrier to construct a multi-functional treatment platform.Objective:The combination of gold nanoparticles and PPD in the basic research of anti laryngocarcinoma treatment can solve the problem of the poor solubility and low bioavailability of PPD.It also provides a new way for the application of gold nanoparticles to clinical treatment.Methods:Hep-2 cells were exposed to different concentrations of PPD,Au NPs and Au NPs containing different concentration PPD for 24 hours.MTT was used to test the relative growth rate of cells.At room temperature liquid phase contact mode atomic force microscopy(AFM)was used to scan Hep-2 cells exposed to control,PPD,Au NPs and combined group.BALB/c mices with 4-6mm tumor were selected and randomly divided into control group,PPD group,Au NPs group and combined group.A subcutaneous injection was given to the tumor in the body.The control group was injected with normal saline.The tumor is stripped then called the mass of the tumor.The difference analysis uses the t-test or the one-way ANOVA method with Graph Pad Prism statistics software.Results:Gold nanoparticles with the concentration of 5mg/L and 10mg/L had no obvious effect on cell growth(P>0.05).Gold nanoparticles with the respective concentrations of 20 mg/L,40 mg/L and 80 mg/L inhibited the growth of Hep-2 cells(P<0.05).The PPD with 10 u M has an effect on the proliferation of Hep-2 cells.After the increasment of PPD concentration(40u M,80 u M,160 u M),it has a certain inhibitory effect on the growth of Hep-2 cells.Commparing with the group of PPD,the combined group were strong inhibitory effective on the growth of Hep-2 cells(P < 0.05),and promoting the inhibition of the small concentration of PPD(10u M,20 u M)on Hep-2 cells growth.The inhibitory effect of PPD on the growth of Hep-2 cells with 160 u M concentration was not significantly different from that of the PPD group(P > 0.05).Hep-2 cells of the control group and PPD group were fusiform,smooth surface,cytoplasm full;The queen,invagination and roughness of Hep-2 cells’ membrane surface which old exposed to gold nanoparticles and combined ones is increased.Compared with the control group,the weight of tumor in each group,gold nanoparticles with concentration of 10mg/L had no significant anti-tumor effect(P > 0.05)and the dose of 40 u M PPD had a certain anti-tumor effect(P < 0.05).The anti tumor effect of the combined group(40u M PPD-10mg/L gold nanoparticles was obvious(P < 0.001).Compared with 40 u M dose PPD group,the antitumor effect of the combined group was more obvious(P < 0.05).Conclusions: 1.Gold nanoparticles had no effect on the growth of Hep-2 cells in a certain concentration range,which could inhibit the growth of Hep-2 cells with increasing concentration.2.PPD with the concentration of 10 u M promoted the growth of Hep-2 cells,and the concentration of PPD in 40 u M,80 u M and 160 u M inhibited its growth.3.A certain concentration of gold nanoparticles can increase the sensitivity of Hep-2 cells to PPD,which can effectively reduce the concentration of PPD drugs and solve the problem of poor solubility and low bioavailability of PPD.4.Gold nanoparticles can play a role in the surface structure of Hep-2 cell membrane.