| Objective:To investigate the status of placental microcirculation and the relationship between placental microcirculation and pregnancy outcomes in patients with preeclampsia by detecting placental tissue Superb Microvascular Imaging and Microvessel Density in patients with preeclampsia.Methods:1 SubjectsSelected from March 2016 to June 2017 in Hebei General Hospital hospitalized preeclampsia women 45 cases(preeclampsia group).Selected the premature birth women 45 cases(control group).There was no significant difference in age and gestational age between the two groups(P>0.05).2 Experimental Methods2.1 SMI Detection All the selected patients were given Aplio 500 ultrasound diagnostic device fixed parameters before delivery.The SMI test was performed by the fixed person.Instruct the patients supine,steady breathing,select the appropriate size of the sampling frame to clearly show the placental microvascular prevail,the color gain adjustment to the small blood vessels can be clearly detected pictures retained.Results using the blind method,The results were independently observed by two senior surgeons.Count ultrasound microvessel density(The number of internal blood vessels per area,n/cm2),the average of which is the ultrasound microvessel density(n/cm2).2.2 MVD Detection Using the CD34 antigen labeled vascular endothelium,showing blood vessels.Placenta after delivery,immediately selected the placenta maternal surface without calcification of the whole layer placenta tissue,which size was about 2cm× 2cm× 2cm.After Fixing by 10% paraformaldehyde embedded in paraffin blocks,All paraffin blocks were cut to a thickness of about 4 μm.The expression of CD34 in placental tissue was observed by using immunohistochemistry(IHC)and Immunofluorescence(IF).In the ×200 field of view,selecting 5non-repetitive fields of view to count,the average number of microvessels is microvessel density(MVD).2.3 Birth Weight The birth weight of all was applied immediately after birth to the same weight scale test that had been calibrated.3 Statistical MethodsSPSS 17.0 statistical software was used to analyze the data.First,the data were tested for normality.The t’test was used If the data conform to the normal distribution.Otherwise,the nonparametric test(rank sum test)was used.The bivariate correlation analysis was performed using Spearman correlation.Taking α =0.05 as the test level,with P<0.05 for the difference was statistically significant.Results:1 SMI Test Results The Ultrasound microvessel density(0.37±0.124)n/cm2 in the placenta of the preeclampsia group was significantly lower than that in the control group,compared with the control group Ultrasound microvessel density(0.45±0.112)n/cm2.There was significant difference between the preeclampsia group and control group(P=0.001).2 MVD Test Results The expression of MVD(104.13±10.008)n/field in the preeclampsia group was significantly lower than that in the control group,compared with the control group MVD(116.44±8.013)n/field.There was significant difference between the preeclampsia group and control group(P<0.001).3 There was a correlation between SMI and MVD in placenta,indicating that there was a positive correlation between SMI and MVD of placental tissue in placental microcirculation(r=0.244,P=0.021).4 There was a positive correlation between MVD and SMI in placental tissue and pregnancy outcome(birth weight)(MVD and birth weight: r =0.454,P<0.01;SMI and birth weight: r=0.350,P=0.001).Conclusions:1 SMI blood flow imaging can reflect the number of placental capillaries,which can be used as a noninvasive assessment of placental capillaries imaging methods.2 SMI blood flow imaging technology can be used in real-time during pregnancy,non-invasive and easy to understand the status of placental microcirculation in preeclampsia patients,preeclampsia pregnancy perinatal outcome prediction has reference value. |
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