The Expression And Regulation Of MicroRNA-1233 In Placenta Of Patients With Hypertensive Disorder Complicating Pregnancy

Objectives To investigate the expression of miR-1233 in placental trophoblast cells and its regulation in the pathogenesis of hypertensive disorder complicating pregnancy.Methods 1 A total of 69 cases undergone caesarean section treatment in obstetrics department of Tangshan Workers’ Hospital during January 2017 to June 2017 were entrolled in the study,the cases including 14 cases of mild preeclampsia,26 cases of severe preeclampsia and 29 cases of normal pregnancy as control group.The expression level of mi R-1233 in different placenta tissue was detected by RT-PCR;Immunohistochemistry and Western blot were used to detected the expression level of HOXB3 protein in placenta tissue of patients with hypertensive disorder complicating pregnancy.2 The JEG-3 cells were cultured,miR-1233 over-expressing were constructed in JEG-3 cells by cell transfection technique,the transfection efficiency of JEG-3 cells was detected by fluorescence microscopy to observe the intracellular green fluorescence;after miR-1233 over-expressing of JEG-3 cells were successfully constructed,MTT assay was used to detect the proliferation ability of JEG-3 cells;Western blot was used to detect the expression level of HOXB3 protein;RT-PCR technique was used to detect the expression level of HOXB3 mRNA.3 SPSS17.0 software was used for statistical analysis and analysis of variance followed by SNK-q test.Results 1 The expression level of mi R-1233 in placenta of patients with hypertensive disorder complicating pregnancy was significantly higher than that of the control group(F=434.425,P<0.05).The expression level of HOXB3 protein was lower in placenta tissues of gestational hypertensive disease,than the control group,and The difference was statistically significant(F=143.721,P<0.05).The results of immunohistochemistry were consistent with the results of Western blot.It showed that compared with the control group,the expression level of HOXB3 protein in the placenta of hypertensive disorder complicating pregnancy was significantly increased(H=21.192,P<0.05).2 After successful transfection,Fluorescence microscopy was used to observe the intracellular green fluorescence,the transfection efficiency of JEG-3 cells was over 70%.After miR-1233 overexpressing JEG-3 cells were successful constructed,the expression of HOXB3 mRNA in the miR-1233 mimics group was significantly lower than that in the blank control group,transfection reagent group,and miR-NC group(F=22.280,P<0.05),while there was no significant difference in the expression of HOXB3 mRNA above the blank control group,the transfection reagent group and the miR-NC group(P>0.05).Compared with the blank control group,the transfection reagent group and miR-NC group,the expression level of HOXB3 protein in JEG-3 cells of miR-1233 mimics group was significantly decreased(F=6.70,P<0.05),while there was no significant difference in the expression of HOXB3 protein above the blank control group,the transfection reagent group and the miR-NC group(P>0.05).Compared with the blank control group,transfection reagent group and miR-NC group,the cells proliferation ability of the miR-1233 mimics group was significantly lower than that of the other three groups(F=16.186,P<0.05).while there was no significant difference in the cell proliferation ability above the blank control group,transfection reagent group and miR-NC group(P>0.05).Conclusions 1 miR-1233 is highly expressed in placental trophoblast cells in hypertensive disorder complicating pregnancy and may be associated with the pathogenesis of hypertensive disorder complicating pregnancy;2 miR-1233 participates in the occurrence and development of hypertensive disorder complicating pregnancy through through negative regulation of the expression of HOXB3.