MiR-372 Inhibits EMT, Invasion And Migration Of Ovarian Cancer Cells And The Possible Mechanism

Objective: To investigate the role and mechanism of miR-372 in epithelial-mesenchymal transition(EMT),invasion and migration of ovarian cancer cells.Methods: miR-372 mimics were transfected into OVCAR3 and A2780 cells by Lipofectamine2000,the expression of miR-372 was detected by RT-PCR.Transwell and cell scratch test were used to detect the invasion and migration ability.The expression of EMT-related proteins N-cadherin and a-SMA protein was detected by Western blotting.Results: The miR-372 expression level in OVCAR3 and A2780 cells was significantly increased after transfection of miR-372 mimics(P<0.05).The invasion and migration ability of OVCAR3 and A2780 cells were significantly decreased after miR-372 mimics transfection(P<0.05).Western blotting results showed that miR-372 overexpression reduced the expression level of EMT-related interstitial proteins N-cadherin and a-SMA proteins.Conclusion: miR-372 overexpression inhibits ovarian cancer cell invasion and migration,and inhibits EMT via inhibiting the expression of N-cadherin and a-SMA protein in ovarian cancer cells.