Study On The Effects Of BPA Exposure During Pregnancy On Neurogenesis In Rats

Objective: bisphenol A(bisphenol A,BPA)Scientific name of 2,2,2 propane)is a common environmental endocrine disruptors,can by disrupting the body or organism itself hormone synthesis,secretion,transport,in accordance with the process,effect and metabolic clearance to or to the human body biological effect on reproductive,nervous and immune systems,etc.Research suggests that BPA on the developing central nervous system neurons,the effect was similar to estrogen,participated in the neurons of the growth,development,maturity and affected the connections between neurons,causing A series of nervous system symptoms.However the dose-effect relationship there is no consistent conclusion exactly,and the study of the maternal BPA exposure during pregnancy are mainly concentrated in hippocampus and thyroid and other viscera,which studies the impact of cerebellar neurons has not been reported see clear,this experiment simulated natural environment,contact via water oral is adopted to establish the model of different concentrations of BPA exposure during pregnancy,by neural development behavior,immunohistochemical method and Westernblot investigate whether maternal exposure to bisphenol A has affected the development of rat cerebellar neurons and its related mechanism.Methods: 41 SD rats were randomly divided into 4 groups(11 high-dose groups),and each group was given A dose of 0,0.5,5.0,and 50mg/kg·day,and the control group was given equal amount of double distilled water.The time of exposure was confirmed to be self-confirmation that the rats were pregnant until delivery,and the weight of the pregnant rats was weighed every day,and bisphenol A was calculated.After the rat was born,it was tested by plane turning,negative geotaxis and rotation bar at corresponding time.Immunohistochemistry was used to detect the expression of microtubule-associated protein MAP2 in microtubule-specific markers in rat cerebellar tissue neurons.The expression of cytoskeletal protein f-actin and g-actin in cerebellar neurons was detected by Westernblot.Detection of actin binding protein: expression of brain development regulation protein Drebrin,filin Cofilin and phosphorylation state p-cofilin;Results:In a flat in turn positive and negative hasten to sex detection: compared with control group,the time needed for each dose group standard(s)are extended,and presents the linear relationship,the longer the exposure dose,the higher the corresponding standard time.In the rotation bar test,compared with the control group,the duration of stay in the exposed dose group was greater than that of the control group.However,during uniform acceleration,the duration of each dose group was shortened and the dose-effect relationship was presented.Immunohistochemical analysis: compared with the control group,the expression of MAP2 at each time point was significantly lower in the high-dose group than in the control group(P<0.05),and the difference was statistically significant.The medium dose group was lower than the control group at PN14(P<0.05),and the difference was statistically significant.There was significant difference between the groups(P<0.05)at PN21.Westernblot: compared with the control group,the expression of f-actin in each dose exposure group decreased,while g-actin was basically unchanged.Compared with the control group,the expression of cofilin in each dose exposure group increased,while the expression of Drebrin and p-cofilin decreased(p <0.05),and the difference was statistically significant.Conclusion: 1: maternal bisphenol A exposure has adverse effects on the balance,movement,coordination and sensation of the mice.2: maternal bisphenol A exposure has adverse effects on the early development of dendritic neurons in the rat cerebellar neurons and presents A dose dependence at A certain level;3: maternal bisphenol A exposure affects the aggregation state of the cytoskeletal protein of the mouse cerebellum neurons,affecting the expression level of actin binding protein.4: the polymerization state of the cytoskeletal protein of the mouse cerebellum neurons exposed by maternal bisphenol A may be related to changes in the expression level of actin binding protein.