Long Noncoding RNA CASC2c Inhibited Cell Proliferation In Hepatocellular Carcinoma By Inactivating The ERK1/2 And Wnt/β-catenin Signaling Pathway

OBJECTIVE:Long non-coding RNAs(lncRNAs)have been shown to play important roles in various tumorigenesis by a large number of evidence,but their biological functions and the underlying molecular mechanisms remain unclear due to the complexity of their sequence and spatial conformation.In this study,the lnc RNA CASC2 c was screened by bioinformatics technology in hepatocellular carcinoma(HCC),and then to further explore the effect of CASC2 c on the HCC cell and specific mechanism.The aim is to provide new test markers of early diagnosis of HCC or potential targets for treatment.METHODS: In this study,36 participants including healthy volunteers and HCC patients were recruited.Quantitative reverse transcription PCR(RT-q PCR)was used to quantitate the expression of CASC2 c in HCC tissues and cells.In different expression of CASC2 c,CCK8 assay was used to test the proliferation ability,transwell assay assay was used to verify the abilities of invasion and migration in vitro.Cell apoptosis assay was used to verify the relation of CASC2 c and cell apoptosis.Western blot was used to detect the effect of CASC2 c on the expression of p-ERK1/2 and β-catenin.Finally,the tumorigenicity experiment in nude mice was used to explore the effect of CASC2 c on the growth of HCC in vivo.RESULTS: Firstly,RT-q PCR analysis showed that CASC2 c was down-regulated in tumor tissues and cell QGY-7703 with HCC.Compared with the negative control group,the proliferation and migration ability of CASC2 c overexpression group were significantly decreased.However,the apoptosis rate of CASC2 c overexpression group was significantly higher than that of the negative control group.Western blot established increased the expression of CASC2 c inhibits the expression of p-ERK1/2 and β-catenin in HCC cell.The tumor growth rate and size of nude mice injected with pc DNA-CASC2c-transfected HCC cells were significantly lower than those of the negative control group.CONCLUSION: This study revealed,for the first time,the importance of CASC2 c in clinical diagnosis and disease progression of HCC.CASC2 c was down-regulated in HCC tissues and HCC cells.CASC2 c inhibited the proliferation,invasion,migration and promoted the apoptosis of HCC cells by inhibiting the expression of p-ERK1/2 and β-catenin.This study demonstrated that CASC2 c could inhibit the growth of HCC in vivo.CASC2 c may constitute a cancer prognostic marker and therapeutic target in HCC.