Effect Of Sorafenib Combined With Cisplatinum On Autophagy And Proliferation Hepatocellular Carcinoma HepG2 Cells In Vitro

Objective To explore the effect of sorafenib combined with cisplatin on cell autophagy and proliferation of liver neoplasms Hep G2 cells in vitro.Method Hep G2 cells were cultured and treated by different concentrations of sorafenib,cisplatin alone or in combination for 24 h.Cell proliferation were detected by CCK8 assay.And the m RNA expression of autophagy-related protein :protein kinase(AKT),human rapamycin target protein(m TOR)and microtubule related protein 1 light chain 3(LC3)were detected by Real-time PCR.Protein expression of AKT,p AKT(Ser473),m TOR,pm TOR(Ser2448),LC3 I,and LC3 II were detected by western blot.Results Compared with using sorafenib and cisplatin along,sorafenib combined with cisplatin had more abilities to depressing the cell proliferation;decreasing the m RNA expression of autophagy-related protein AKT,m TOR and increasing that of LC3(Single factor analysis of variance and SNK-q test,F=181.32~282.10,q=6.5~71.36,P<0.05);and decreasing the phosphorylation of autophagy-related protein AKT,m TOR and increasing that of LC3(F =1236.00、748.00,q =58.70、74.70、73.70;F =4104.00,q =117.30、131.30、132.70,P<0.05).Compared with the control group,sorafenib along could also decrease the phosphorylation of autophagy-related protein AKT,m TOR and increase that of LC3(q=14.60、11.20,q=15.4,P<0.05),but cisplatin along made no difference(P>0.05).Conclusion Sorafenibcombined with cisplatin can effectively induce cell autophagy and depressing the cell proliferation through PI3K/AKT/m TOR signal pathway.