The Stem Cell Character And Invasiveness In 3D Culture Of Tumor Tissue

Most cancer patients will develop resistance mechanisms after several months of chemotherapy.In clinical practice,the combination of different drugs is used to increase the efficacy of chemotherapy and reduce adverse drug reactions in order to achieve better therapeutic effects.Among them,glioma(GBM)is characterized by strong invasiveness and recurrence,and inhibiting its invasiveness may play an effective role in the treatment.In this paper,three-dimensional cultures of tumor tissue were used in combination to observe whether the drug-resistant cells had the expression of stem cell marker CD44;it was found that the expression of CD44 in tumor tissue increased after in vitro administration,and the preliminary exploration of the relationship between CD44 and drug-resistant cells.Correlation.A small proportion of drug-resistant cells in breast and pancreatic cancer have CD44 expression.In this dissertation,the effect of ITE on the invasion of glioma cells was observed in mouse brain slices in vitro.It was found that ITE can inhibit the invasion of glioma cells.The efficacy of the combined use of the small molecule compound ITE with 5-fluorouracil and paclitaxel was studied in a mouse model of orthotopic glioma.It was found that combination therapy did not increase the survival rate of mice,and the specific causes were worth further investigation.Immunofluorescence was used to detect mouse glioma after ITE treatment.It was found that there was also a small amount of CD44 expression in the dosing group.Conclusion: A small amount of drug-resistant cells express CD44 in human pancreatic cancer tissues and breast cancer tissues.ITE can inhibit the invasion of glioma cells.There is expression of CD44 in mouse glioma model after ITE treatment.