Verification Of Proteomics Markers Of Asymptomatic Symptoms Of HIV/AIDS In Chinese Medicine Based On Drug Intervention

Objective AIDS asymptomatic period is an important period of intervention of Chinese and western medicine,and it is of great significance to explore the syndromes and characteristics of traditional Chinese medicine in order to improve TCM syndrome differentiation and improve the curative effect.This study was based on the early use of i TRAQ technology screening of AIDS asymptomatic heat poisoning syndrome patients with significant differences in protein,and the application of Western Blot technology and then further clinical validation,hoping to obtain AIDS asymptomatic Chinese medicine hot toxic The characteristic expression of the protein.On the basis of this,the patients were treated with Ai Fukang capsule combined with HAART intervention to observe the clinical curative effect of the intervention drug,the change trend of the characteristic protein expression of the asymptomatic symptoms of AIDS,And to explore the significance of the evaluation of the efficacy of the characteristics of the preliminary exploratory study.Methods Collected for 0 weeks and treated with Ai Fukang Capsule combined with HAART for 48 weeks.The serum samples were collected by Western Blot technique.The serum samples were collected from the healthy control group.The serum samples were collected by Western Blot.C3,C5,GPX,Apo D,Cytokeratin10,Fibronectin protein were detected.And then use the image J software to analyze the gray value.The gray value of the target protein obtained from each lane was compared with the corresponding albumin gray value,and the relative gray value was obtained.Then,the healthy control group,The relative gray values of the patients in the asymptomatic period and the 48 weeks of treatment were introduced into the Graph Pad Prism 5 software.Statistical analysis was performed using IBM SPSS Statistics V20.0 software.Count data using the Fisher exact probability method.Mean ± standard deviation(± s)to represent the normal distribution data.And with P <0.05 standard as a difference standard.Results 1.the AIDS drug in patients with toxic heat accumulation syndrome symptoms before with the healthy control group were compared,C3 protein in AIDS symptoms toxic heat accumulation syndrome before treatment expression,the difference was statistically significant(P<0.05);using i TRAQ technology with the pre screened protein results.2.the AIDS drug in patients with toxic heat accumulation syndrome symptoms before with the healthy control group were compared,C5 protein in AIDS symptoms toxic heat accumulation syndrome before treatment expression,the difference was statistically significant(P<0.05);using i TRAQ technology with the pre screened protein results.3.AIDS symptoms of drug toxic heat accumulation syndrome patients after 48 weeks compared with before treatment,CD4+,CD8 tended to increase steadily,the difference of CD4 was statistically significant(P < 0.05),CD8 was not statistically significant.4.AIDS symptoms of drug toxic heat accumulation syndrome patients after 48 weeks compared with before treatment,viral load Significantly decreased,and the viral load difference was statistically significant(P < 0.05).5.The expression of C5 protein in the asymptomatic period of AIDS was significantly higher than that in the control group(P <0.05),and the difference was statistically significant(P <0.05).Compared with the healthy control group,the expression of C5 protein in the asymptomatic period of AIDS was up-regulated at 48 weeks,and the difference was not statistically significant(P <0.05).6.The expression of C3 protein in the patients with asymptomatic symptoms of AIDS was significantly higher than that of the control group(P <0.05),and the difference was not significant(P <0.05)).After comparison with healthy control group,the expression of C3 protein in the asymptomatic period of AIDS was statistically significant(P <0.05),and there was no significant difference between the two groups(P <0.05).conclusion 1.The characteristic expression of protein 1,this study shows that C3 and C5 be used as AIDS symptoms may be related to toxic heat accumulation syndrome,immune function and inflammatory reaction involved.Oxidative stress and immune response.2.C5 expression quantity changes with AIDS symptoms toxic heat accumulation syndrome clinical curative effect of traditional Chinese medicine syndrome differentiation trend was consistent,with clinical evaluation for the treatment of AIDS patients with toxic heat accumulation syndrome of significance.