Study About Hematopoietic Regulation And Its Mechanisms Of Taishan Panshi Powder On Myelosuppressed Mice

Objictive:And bone marrow hematopoietic function in patients with tumor after chemotherapy severely damaged,with reduced platelet and white blood cells and other related to bone marrow suppression phenomenon is obvious.Enhance,fixed number of year of the patients with tumor after chemotherapy survival,and quality,clinical application,in order to better explore put,chemotherapy to reduce side effects of traditional Chinese medicine compound preparations is a feasible way.This lab to select the present clinical application in Taishan stone powder,on the mechanism of tonifying qi and blood,in order to get the party to influence hematopoiesis regulation principle of experimental data such as support,and further provide strong support for its clinical application.Methods:Making bone marrow suppression model mice,application of hematopoietic bone cells,bone marrow biopsy and immunohistochemical,peripheral blood cell count,and flow cytometry technology,and hematopoietic progenitor cells from bone marrow cells and peripheral blood cells of survival,proliferation and cell cycle,multi-angle to taishan rock to inhibit mice bone marrow hematopoietic effect and its mechanism were discussed and studied.Results:1 Taishan stone powder can obviously promote bone marrow inhibition of mouse bone marrow nucleated cells(BMC),hematopoietic tissue area,the number of peripheral blood cells(WBC),red blood cell(RBC),hemoglobin(Hb)content and increase the number of platelets(PLT),among them,the best dose group.2 Taishan stone powder in the body can promote red blood,bone marrow suppression mice grain,giant nuclear three-line hematopoietic stem/progenitor cells in vitro cultivation of colony formation,and each has its characteristics.3 Taishan stone powder can cause the inhibition of mouse bone marrow hematopoietic stem/progenitor cells into the cell proliferation cycle,reduce G0 / G1 phase cell proportion,rising rates of S phase and G2 / M phase cells,Taishan stone powder can increase bone marrow cell proliferation index(PI).Conclusion:Taishan stone powder can improve the hematopoietic function of bone marrow suppression mice,have promote model groups like mice peripheral blood and bone marrow as recovery;Promoting hematopoietic stem/progenitor cell proliferation and differentiation,and accelerate hematopoietic stem/progenitor cells in G0 / G1 phase to S phase cells,S phase cells to the G2 / M phase transformation;The compound through the above ways to enhance the recovery of bone marrow hematopoietic function of bone marrow suppression mice.