Research On Serum Fingerprint Of Lichongshengsui Decoction

LICHONGSHENGSUI Decoction is a Chinese herbal prescription.provided by Professor Wang Xiuxia and baesd on traditional Chinese medicine theory,commonly used to treat ovarian cancer.However,the constituents absorbed into the blood after oral administration of LICHONGSHENGSUI Decoction are difficult to determine and thus remain unclear.Here,we report the application of an accurate high performance liquid chromatography,ultra performance liquid chromatography for developing serum fingerprints and detecting the compounds of LICHONGSHENGSUI Decoction in vivo,aiming at providing foundations for further research of its active compounds and mechanism in vivo.First,the use of high performance liquid chromatography technology,by examining the chromatographic conditions(including the mobile phase,detection wavelength,column temperature,flow rate,running time,etc.),serum treatment,blood collection point,the precision,stability,repeatability experiments established analytical method LICHONGSHENGSUI Decoction serum HPLC fingerprint chromatographic conditions were:equipment for the Waters HPLC and diode array detector;column was DIKMA C18 column(5μm,250 mm × 4.6 mm);mobile phase acetonitrile as A,B water,gradient elution program was:0-10min,95%-80%A;10-45min,80%-45%A;45-60min,45%-10%A;60-80min,10%A；detection wavelength of 210nm,the column temperature was 30℃,the injection volume was 10μL,the flow rate of 1mL/min.Acetonitrile serum treatment method,2h blood after administration.Precision,stability,repeatability test each chromatographic peak retention time and peak area RSD were less than 3%.Similarity batches fingerprints were above 0.9,were identified 28 common peaks,which peaks on the 4th of ginseng peak number Rg1,10 as icariin peak on the 19th to treasure beans glycosides I,26 peak β-elemene.Then,using U ltra Performance Liquid C hroma to graphtechno logy,by examining the chromatographic conditions(including the mobile phase,column temperature,flow rate,run time,sample volume,etc,),the precision,stability,repeatability experiments established a serum UPLC analysis LICHONGSHENGSUI Decoction fingerprints,chromatographic conditions:DIKMA C18 column(1.8 μm,50 mm × 2.1 mm);acetonitrile mobile phase was a,B water,gradient elution is:0 2min,95%A;2-4min,95%-85%A;4-15min,85%-50%A;15-16min,50%-44%A;16-20min,44%-20%A;20-31min,20%-0%A;detection wavelength of 210nm,the column temperature was 30℃,the injection volume 3μL,a flow rate of 0.3mL/min.Precision,stability,repeatability test each chromatographic peak retention time and peak area RSD were less than 3%.Similarity batches fingerprints were above 0.9,were identified 29 common peaks,where the 3rd peak peak ginseng Rgl,7 number icariin,14 peak as curcumol,the 15th peak treasure beans glycosides I,the 24th peak β--elemene.The method established is rapid and sensitive for detecting serum UPLC fingerprint of LICHONGSHENGSUI Decoction,which will be a new way for investigation of active compounds in LICHONGSHEN GSUI Decoction.