| Objective:To explore clinical significance of uncoupling protein-2 in the clinical diagnosis and explore whether the protein is associated with gastric cancer staging and gastric cancer cell classification,and indirectly,to know whether the protein concentration is associated with the prognosis of gastric cancer.Methods:①A total of 102 patients were recruited from the Mian yang Central Hospital,Sichuan Province,between January 2014 to March 2016.All patients were diagnosed by gastric endoscopy and pathological examination(This method is considered the gold standard for diagnosis of gastric cancer).There were 55 males and 47 females with the mean age of 60 years(range:28-89 years).The patients with diabetes mellitus,hyperlipidemia,severe obesity,other malignancies(such as colon cancer,lung cancer,liver cancer etc,but not including distant metastasis of gastric cancer),or cardiovascular disease were excluded.Furthermore,those patients were also excluded if the patient’s cardiopulmonary function is very poor as a result that they can not undergo surgery.In our experiment,110 healthy volunteers who received endoscopy and pathologic examination were also recruited as controls(there are 58 males and 52 females,the mean age of 58 years with a range 29-88 years).All healthy subjects were not confirmed gastric cancer and other organic stomach trouble,while not associated with diabetes,hyperlipidemia,severe obesity,cardiovascular disease and other malignancies(such as colon,lung,liver,etc).② Serum UCP-2 concentrations were detested using a enzyme-linked immunosorbent assay(ELISA)method in gastric cancer patients before operation and healthy control group.All gastric cancer patients accept the same or similar surgical approach.③The venous blood samples from patients with gastric cancer before surgery were grouped according to tumor invasion depth(T stage),regional lymph node metastasis(N stage),distant metastasis(M stage).At the same time,the venous blood samples from patients with gastric cancer before surgery were also grouped according to different tumor cell differentiation(low,medium,well-differentiated).We analyzed the difference of UCP-2 concentration between each two groups and determined whether there were statistical significance.At the same time,We analyzed the difference concentration of UCP-2 between gastric cancer patients and normal control group,and analyzed the difference of UCP-2 concentrations between preoperative group and postoperative group to understand whether it has statistical significance.Results:Statistical analysis showed that UCP-2 levles was not a normal distribution,so numerical values were reported as median with a minimum(Min)and maximum(Max).①Before surgery undergoing gastric cancer,the Min,Max,and median of UCP-2 levels in 102 patients were 1.05 pg/ml,41.79pg/ml,and 10.12 pg/ml,respectively;The Min,Max and median of UCP-2 concentrations in 110 healthy subjects were 1.23 pg/ml,227.16pg/ml,and 14.19 pg/ml.The two independent samples nonparametric Mann-Whitney test showed that the concentration of UCP-2 in the group of gastric cancer was lower than in the normal control group(Z=-6.335,P=0.000.②The preoperative patients with gastric cancer were groups according to different clinical staging,and were compared the differences of UCP-2 concentrations between each two groups.Statistical analysis showed that there were no significant differences(X2=0.786,P=0.853)were found in UCP-2 concentrations between each two groups.③The preoperative patients with gastric cancer were groups according to the tumor cell invasion depth(T stage),and were compared the differences of UCP-2 concentrations between each two group.The results for statistical analysis also showed that there was no significant difference in UCP-2 concentration in each two groups was observed(X2=3.948,P=0.267).④The preoperative patients with gastric cancer were grouped according to the tumor regional lymph node metastasis(N stage),and were compared the differences of UCP-2 concentrations between each two groups.The results for statistical analysis showed that the UCP-2 concentrations between each two groups didn’t exist significant differences(X2=0.117,P=0.990).⑤ We grouped the preoperative patients with gastric cancer according to the tumor distant(M stage),and compared the differences of UCP-2 concentrations between each two groups.The results for statistical analysis showed that the UCP-2 concentrations in each two groups didn’t exist significant differences(Z=-1.395,P=0.163).⑥According to the tumor grade,we grouped the preoperative patients with gastric cancer and compared the differences of UCP-2 concentrations between each two groups.Statistics showed that the of UCP-2 concentrations in blood samples in each two groups didn’t exist significant difference(X2=1.570,P=0.666).Conclusions:①The concentration of UCP-2 in the venous blood samples of patients with gastric cancer is lower than those of the healthy controls.Serum UCP-2 may become a new serological indicator,which provide the evidence for gastric cancer screening,diagnosis.② The concentration of UCP-2 in the venous blood samples in patients with gastric cancer has not been associated with the tumor grade or stage.Therefore,the protein is not as an indirect index to assess the prognosis of gastric cancer. |
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