The Clinical Study Of Tonify The Kidney And Active Blood Prescription Affects The PGE2 And COX-2 Cytokines Levels Of ACA(+)Patients With Recurrent Spontaneous Abortion

Objective:The study based on clinical trials observes the effect on Tonify The Kidney and Active Blood Prescription treating the recurrent spontaneous abortion patients’ PGE2 and COX2 cytokines levels of ACA(+)with the PGE2 and COX-2 cytokines as outcome measures.Adopt real-time quantitative fluorescent PCR(RT-PCR)and enzyme-linked immunosorbent assay(ELISA)experiment method to differ the changes of APGE2 and COX2 levels in RSA patients.Discuss the connection of PGE2 and COX-2 cytokines with ACA(+)RSA,and the Tonify The Kidney and Active Blood Prescription of tocolysis mechanism,provide a basis for the study of anti-cardiolipin antibodies recurrent abortion signaling pathways.Methods:This experiment consists of two parts,the data obtained after the analysis of the results are discussed:Experiment 1:select the research object as the group of the.15 cases of normal abortion were collected,and 15 cases of ACA(+)RSA were aborted.The content of PGE2mRNA and COX-2mRNA in the two groups were determined by RT-PCR method.Experiment 2:the selected subjects were serum group.Collection of out-patient routine antenatal patients in 10 cases(6-8 weeks pregnant)and ACA(+)RSA given Bushen Huoxue Decoction Treatment of 4 weeks of pregnancy were 10 cases(6-8 weeks pregnant).In group and group 4 weeks after collecting venous blood of two groups of patients,with the level of PGE2 and COX-2 were detected by enzyme-linked immunosorbent assay(ELISA)were detected in serum.Result:1.ACA(+)patients with recurrent spontaneous abortion PGE2 mRNA and COX-2 mRNA contents in deciduas are lower than Normal abortion patients2.The amount of PGE2 and COX-2 in the serum of ACA(+)RSA patients were lower than those in the normal serum group;3.The expression levels of PGE2 and COX-2 in serum of ACA(+)RSA were increased after 4 weeks of treatment with oral administration of the kidney and promoting blood circulation.Conclusion:1.The low expression levels of PGE2 and COX-2 in serum and in the lower level of ACA were related to ACA(+)RSA,suggesting that it might be one of the mechanisms of recurrent spontaneous abortion.2.Reinforcing kidney and activating blood prescription can effectively increase the level of PGE2 and COX-2 in the serum of ACA(+)patients,improve the immune status of the imbalance of the body,prevent thrombosis,and provide a solid foundation for the normal pregnancy.