Extraction,Isolation And Bioactivities Of Active Ingredients From Trichosanthes Kirilowii Peel

Diabetes mellitus has become the highest mortality rate of chronic diseases after cancer and cardiovascular disease.Nowadays,study on antioxidant drugs is a hot topic.Many researches have shown that Trichosanthes kirilowii with obvious therapeutic efficiency in hypoglycemic and antioxidant.Therefore,it's very important and necessary to research of the active ingredients,extraction technology and biological activity of Trichosanthes kirilowii peel because of its academic value and practical significance.Trichosanthes kirilowii peel was extracted by ultrasonic assisted enzymatic extraction method,using the content of total sugar as index.Through the single factor design and response surface experiment,the optimal extraction technology with ultrasonic power of 570W for5.52min,the enzymolysis time of 75min at pH4.76 solution and maintained at 50~oC,the enzyme amount of 0.7%(W/W)and the liquid-solid ratio of 9.90 was obtained,which showed the yield of T.kirilowii peel polysaccharides was 22.50%.Meanwhile,the yield in confirmatory experiment was 22.45±0.38%.A variety of polysaccharide purification technologies and methods were utilized to purify the polysaccharide of Trichosanthes kirilowii peel.The structure of polysaccharide was characterized by infrared spectroscopy(IR)and nuclear magnetic resonance spectroscopy(NMR)and the molecular weight,monosaccharide composition of the polysaccharide was also determined.The results showed that the molecular weight of Trichosanthes kirilowii purified polysaccharide(TKPP)was 11749Da,mainly composed of D-mannose,L-rhamnose,D-glucose and D-galactose.The?-glucosidase inhibitory and antioxidant experiments showed that TKPP has the potential of develop to hypoglycemic drugs and antioxidants agents.The extracts of Trichosanthes kirilowii peel in dichloromethane phase were isolated and characterized by column chromatography separation technology,IR and NMR.Six compounds including?-spinasterol,palmitic acid,methyl palmitate,vanillic acid,gentisic acid and oleanolic acid were obtained.