Study Of MEST Gene Methylation And Its Correlation With Glycometabolism And Lipid Metabolism Disorder In Gestational Diabetes Mellitus Patients And Newborn

ObjectiveTo investigate the relationship of maternal and neonatal serum glucose and lipid levels,MEST gene promoter methylation,glycometabolism and lipid metabolism disorder in gestational diabetes mellitus patients and newbornMethodsA case-control study included 59 normal pregnant women and 71 pregnant women with GDM from Hangzhou First People’s Hospital during Sep 2013 to Dec 2013.The level of maternal and neonatal serum Fasting Blood-glucose,Fasting insulin,Triglyceride,Total Cholestrol,High Density Lipoprotein Cholesterol,Low Density Lipoprotein Cholesterol,Free Fatty Acids,Apolipoprotein A1,Apolipoprotein B were measured.Hemoglobin A1c of GDM group were also measured.While the maternal weight、height、weight gain during pregnancy and newborn birth weight were recorded.HOMA insulin resistance index were also calculated.Maternal fasting venous blood were obtained before delivery,umbilical vein blood were also collected immediately after birth,then Genome DNA were extracted.Methylight was used to compare the promoter methylation levels of MEST gene in maternal and cord blood.Resuits1.Compared with the control group,BMI before pregenancy of GDM group pregnancies were obviously higher.Weight gain during pregnancy of GDM group pregnancies were significantly lower than the control group.(P<0.05)2.the maternal HOMA IR,TG of GDM group pregnancies were obviously higher(P<0.05).While maternal ApoAl were significantly lower than the control group.(P<0.05)3.Mean neonatal weight showed no significant difference between two groups.(P>0.05)4.The neonatal serum INS of GDM group were significantly higher than control group(P<0.05)5.BMI before pregenancy,BMI before Dilivery in both groups showed positive correlation with neonatal weight.(P<0.05)6.Maternal HbAlc of GDM group showed positive correlation with neonatal weight.(P<0.05)7.The neonatal serum INS of GDM group showed positive correlation with neonatal weight.(P<0.05)8.The MEST gene promoter showed significantly lower methylation levels in both maternal and neonatal of GDM group.(P<0.05)9.The maternal MEST gene promoter methylation levels were significantly higher than neonatal MEST gene promoter of GDM grouplO.The maternal MEST gene promoter methylation levels showed negative correlation with maternal TG and LDL-C of GDM group.The neonatal MEST gene promoter methylation levels showed positive correlation with BMI before pregenancy and weight gain during pregnancy of GDM group.The neonatal MEST gene promoter methylation levels showed negative correlation with BMI before Dilivery and maternal TG levels,(P<0.05)ConclusionThe MEST gene promoter showed significantly lower methylation levels in both maternal and neonatal of GDM group.MEST DNA methylation levels was affected by Epigenetics and intrauterine hyperglycemia and hyperlipidemia.