The Influence Of High Fat Diet On The Expression Of Rats’ HPGA Related Genes And The Intervening Effect Of Maturation NO.2 On It

Objective To research the influence of high fat diet on the female rats’expression of hypothalamus GnRH mRNA,Kiss-1 mRNA,GPR54 mRNA and pituitary GnRH-R mRNA,and the intervention mechanism of Maturation NO.2 on it.Methods Choosing 30 female SD rats of 21 days old,randomly divided them into 3 groups,namely high-fat group,control group and CHM group,10 in each group.Control group was fed with standard feed.High-fat group and CHM group were fed with high-fat feed.At the same time,CHM group was gavaged with Maturation N0.2,while the others were gavaged with normal saline.When the 10 rats in the high-fat group appeared to have vaginal opening,all the rats were going to be executed to obtain the hypothalami and pituitaries.The relative expression of hypothalamus GnRH mRNA,Kiss-1 mRNA,GPR54 mRNA and pituitary GnRH-R mRNA were detected by RT-PCR.Result The relative expression of hypothalamus GnRH mRNA,Kiss-1 mRNA,GPR54 mRNA and pituitary GnRH-R mRNA:high-fat group was much higher than control group(P<0.05).CHM group was lower than high-fat group,but there was no significant difference between them(P>0.05).Conclusion Long-term high-fat diet can cause the female rats’ function of HPGA to start ahead of schedule.Maturation NO.2 has no significant inhibitory effect on the precocious puberty caused by high-fat diet.