| Background and objective:Renal ischemia-reperfusion injury(IRI)is one of the leading causes of acute kidney injury(AKI)and often occurs in urological surgery and cardiac and macrovascular surgery,which is also associated with recent and long-term prognosis.How to reduce the occurrence of renal IRI,to promote the recovery of renal function as soon as possible,is the focus of the current research and difficult.As a new noninvasive preventive measure,remote ischemic preconditioning(RIPC)has been extensively studied.The aim of this study was to investigate the effect of RIPC on renal IRI and to explore the role of COX-2/mPGES-1/PGE2 in this process.Methods:Sprague-Dawley rats were randomly divided into three groups following right-side nephrectomy:Sham-group(only free left renal pedicle),IRI-group(eft renal artery occlusion for 45 minutes),RIPC-group(pretreated with 4 cycles of 5-minute ischemia and 5-minute reperfusion of the hind limb 24 h before clamping),RIPC+Vehicle group(RIPC treatment with the injection of EP4 receptor agonist),RIPC+Vehicle group(RIPC treatment with the injection of saline).Serum creatinine(SCr),blood urea nitrogen(BUN)and renal tubular injury were measured at 12,24,and 72 hours after operation.The expression of cyclooxygenase-2(COX2),membrane associated prostaglandin E synthase-1(mPGES-1)and prostaglandin E-2(PGE2)were also detected.Results:Rats treated with RIPC before ischemia revealed significant improvements in renal function and morphology(p<0.05).Additionally,the expression of COX-2 and PGE2 were obviously decreased in RIPC group than those in the IRI group(p<0.05),however,the content of mPGES-1 remained no significant change(p>0.05).The renal protective role of RIPC was significantly weakened after the use of EP4 receptor agonists.Conclusion:The results suggested that RIPC could attenuate renal IRI and improve kidney function.The COX-2/PGE2 pathway may play a critical role in this process. |
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